Development of a sensor-connected in vitro neuromuscular co-culture system may provide a useful tool for interpreting mutual signal transfers between the cell species and for future applications in drug screening. A glass-based, autoclavable, micro fluidic [...]
An automated nanoparticle based microfluidic platform called GRAVI™ will be presented here as a novel benchtop robotised system for fast and automated ELISA analysis in low volumes. This system can handle samples and reagents from [...]
Isolation of White Blood Cells (also called “WBC”) from whole blood is a first very important step for many in vitro assays. One currently accepted technique for WBC separation, referred to as the conventional manual [...]
A polymer paraboloid microchip is utilised for performing fluorescence detection in point-of-care devices, employing a mass producible and effective strategy. The chip consists of a single piece of moulded plastic containing nine parabolic elements, with [...]
We report a fully integrated, one-step Lab-on-a-Disc for detecting target protein from whole blood utilizing centrifugal microfluidics, so called “Immunoassay Lab-on-a-Disc”. The design principle is the microbead-based sandwich ELISA on the Disc. The microbeads are [...]
In recent years, microfluidics has demonstrated novel methods of culturing and studying cells. Within well-defined volumes comparable to the sample of interest, media, nutrients or toxins may be precisely targeted to individual cells via diffusion [...]
This paper presents a novel method of DNA extraction from whole blood using time varying magnetic field. The novelty of this chip is that both mixing and separation steps are performed in a single chamber [...]
Glucose and lactate are the most important metabolites which characterize physiological state and the metabolic activity of living cells. Consequently, development of a stable glucose biosensor is an interesting and important research topic for medicine [...]
Hierarchical nanoporous materials afford the opportunity to combine the high surface area and functionality of nanopores with the superior charge/discharge characteristics of wider transport channels. In the present paper we optimize the apertures and spacing [...]
Mixing, particle transport, and lysis of biological cells can be performed in microfluidic chips by actuators based on the controlled motion of a gas-liquid interface. There is strong motivation to develop practical and tractable computational [...]
The following work presents the fluidic and electrical characterization of a 3D electrodes array to be used in high throughput and high efficiency Carbon Dielectrophoresis (CarbonDEP) applications such as multi-stage filters, continuous particle enrichment and [...]
In this work, we introduce the novel technique of in-chip drop-on-demand, which consists in dispensing picoliter to nanoliter drops on demand directly in the liquid-filled channels of a polymer microfluidic chip. The technique involves a [...]
The demand for specific tools intended for the deposition of small amount of material on a nanoscale at predefined location is continuously increasing. Several dispensing tools for liquid, such as ink-jet or pin-spotting heads have [...]
Printing technology is considered to be a key technology even in the field of electronics, materials processing, and bio applications. Inkjet printing is an interesting patterning technique for electronic devices because it requires no physical [...]
In this work, we introduce the parallel flow control (PFC) configuration shown in Fig. 1 to address these key interfacing and flow control issues. PFC uses flow in a pressure-driven micro-channel, which interfaces with the [...]
We present a novel CMOS/MEMS microfluidic device that enables the controlled production and redirection of streams of picoliter-sized droplets at frequency rates in the hundreds of kilohertz range. This device consists of a pressurized reservoir [...]
We have designed a micro system - suitable for parallelization - to culture cells three dimensional. Our system consists of the CellChip, a body and a pneumatically driven micro pump.The aim of our system is [...]
We report a microdispenser that can meter and mix sub-nanoliter liquids in a discrete quantity. Our device is based on the structural stopping valve used in passive dispensers. A vent channel was proposed, designed for [...]
In recent years, the application of microelectromechanical systems (MEMS) technology on biomedical fluidic analysis has progressed rapidly. By minimizing the system, the time of analysis and reaction can be effectively shortened. Also, the quantities of [...]
We present the theory, simulations and experiments for discrete drop mixing in microfluidic systems. The generalized analytical solutions have been derived and confirmed by simulations and on-chip experiments. The discrete drop mixing occurs in three [...]
Magnetic nanoparticles are finding increasing use in a broad range of bioapplications, primarily as carrier particles for biomaterials such as cells, proteins, antigens and DNA. The use of biofunctional magnetic particles enables selective immobilization and [...]
We use a spatially periodical electrode array in a fluid chamber to generate a high gradient electric field. Neutrally buoyant poly alpha olefin particles in corn oil in the chamber are transported to the lower [...]
The manipulation of molecules is a crucial prerequisite for the emerging field of molecular nanobiotechnology. This paper reports measurements that characterize the immobilization of 48 kilobase-pair lambda DNA onto lifted-off microelectrodes by high-voltage and high-frequency [...]
We present a multi-stage, multi-frequency carbon dielectrophoresis (CarbonDEP) device for high throughput filtering and separation applications in the health care and biotechnology fields. CarbonDEP refers to the use of carbon electrodes to induce DEP. Up [...]
This paper presents lateral-driven continuous dielectrophoretic (DEP) microseparator for separating red and white blood cells suspended in a highly conductive dilute whole blood. The DEP microseparators enable the separation of blood cells with continuous operation [...]
In current advances of biotechnology, various models of introduction of DNA into cells to alter their gene expression have important biomedical and bioengineering applications. However, the molecular mechanisms underlying how DNA’s cross cell and nuclear [...]
Mice brain atlas and 3D reconstructed morphology serves a great deal od real time monitoring of pharmacological properties of drugs. Iron oxide based nanoparticles paved the way to get higher resolution and contrast using MRI [...]
Single molecule detection has been a hot point problem of science and research, classic dye fluorescent marking technology has a many defects, for example fluorescent power low, fluorescent instability , and so on. Fluorescent quantum [...]
Polystyrene bead and electrospun polystyrene nanofibers are employed as subjects of study in this protein biomarker sensor. The study is conducted to compare the sensitivity and efficiency of the polystyrene nanofibers and microspheres as protein [...]
Robust diagnosis of a disease can be accomplished by reliable detection of multiple protein biomarkers. Traditional assay methods for protein detection such as enzyme-linked immunosorbent assay (ELISA) have several limitations – need for use of [...]
A combinational method has been developed for ultra-sensitive detection of E. coli. The method combined biological, nano-fabrication and electrochemical techniques to achieve highly specific and ultra-sensitive detection of bacterial pathogen. E. coli K12 was used [...]
The goal of this research is to be able to develop a device that would be able to detect and diagnosis pancreatic cancer at bedside. The key criteria to be addressed are the cost, portability, [...]
We report the synthesis and modification of polyacrylic acid (PAA) monolayers in nanoporous alumina membranes. PAA was adsorbed onto the membrane surface using solution self-assembly to provide 0.5 -1 nm films that are robustly bound [...]
A 2D crystalline nanoarray of organothiols has been used for enhancing the detection sensitivity of Surface Plasmon Resonance-based biosensor. In particular, an IgG/AbIgG immunoreaction has been monitored by Surface Plasmon Resonance imaging on nano-patterned surfaces [...]
Hollow Fiber Diafiltration (or Hollow Fiber Tangential Flow Filtration) is an efficient and rapid alternative to traditional methods of nanoparticle purification such as ultracentrifugation, stirred cell filtration, dialysis, or chromatography. Hollow Fiber Diafiltration can be [...]
The overall object of this paper is a systematic study of gaseous flows and thermal transport in two-dimensional micro- and nano-channels using direct simulation Monte Carlo (DSMC) method. The flows are often encountered in MEMS, [...]
Experimental measurement of fluid flow and diffusion through nanoscale channels is important both for determining how classical theories of fluid dynamics apply at very small length scales and with a view to constructing practical nanofluidic [...]
The physics of micro/nanofluids has become an area of intense interest both scientifically and technologically. The long-range nature of viscous flows and the small dimension inherent in confined spaces imply that the influence of boundaries [...]
In this paper MD simulation has been performed to study the behavior of nano-sized non-polar argon droplets spreading on a solid surface. Three different sizes of the nano-droplet have been examined with the diameters of [...]
Electrohydrodynamic Atomization (EHDA) is a method to disperse liquid jet into micro- droplets where an electrical field is applied to control the breakup characteristic of the liquid jet. Recent studies show that EHDA method is [...]
Microfluidic systems have been developed and used for high throughput cell-based assays successfully. However, these systems require complicated peripherals and a computer to control the flow, which limits their adoption by biologists. Here, we propose [...]
, Chappell S.
, Errington R.J.
, Furon E.
, Menachery A.
, Morris D.
, Pethig R.
, Smith P.J.
, Wiltshire M.
, Bangor University, UK
In this study, the ability of Dielectrophoresis (DEP) to recognize phenotypic variants of small cell lung cancer (SCLC) cells has been tested. The phenotypic variations of the SCLC cells are associated with the cell-surface decoration [...]
Numerical simulation is performed of motion and cluster formation of particles in a flowing suspension of particles in corn oil through a 60x120x3.0mm chamber with a spatially periodical electrode array. The chamber is rotated to [...]
In this study, we demonstrate a new passive micromixer, a self-vortical micromixer, and integrated this micromixer into a micro-DMFC.
We have developed pressure driven micro pump. They can deliver gas and liquid. That is an absolute advantage of the scale of this pump. Our pump is designed for integration in a multi well plate [...]
This work adapts DC-DEP to a medical diagnostic application of sorting blood cells. Initial efforts focus on achieving separation and collection of red blood cells based on their positive ABO blood types. A significant separation [...]
The effect of particle characteristics and polymer rheology on the fabrication of sintered ceramic microarrays using powder injection modling is examined. The impact of material composition on microfeature resolution and defect avoidance during processing was [...]
Many technologies require the understanding of conduction and electrical transport mechanisms through nanostructures. Previous research has revealed unusual and enhanced conduction properties in pores whose width is significantly less than 1 m over a range [...]
We recently demonstrated that long metal nanoneedles grown on atomic force microscope (AFM) probes can give very clear measurements of the surface tension, evaporation rate and viscosity of simple liquids. However, in the case of [...]
The treatment of cerebral aneurysms with a minimally invasive porous stent has recently been proposed to prevent rupture and favor coagulation mechanism inside the aneurysm. But, an irregularly shaped and wide-necked aneurysm makes current stent [...]
Next generation fluidic micro-nano devices will require precise control of force fields within a device and their interaction with a biological sample. Current force field designs use mechanical structures such as cantilevers, optical fields e.g. [...]
Flows containing long-chain polymers are viscoelastic on macroscopic length scales, but in microscale and nanoscale flows hybrid fluid-particle approaches are more suitable. Such an approach would enable more complex predictive modeling, including polymer-wall interactions like [...]
We will report on our recent advances made in predicting this class of flows with our CMFD code TransAT (www.ascomp.ch), using the Level Set approach as an “Interface Tracking Method”. Our code incorporate phase-change capabilities, [...]
A multicomponent lattice Boltzmann model is used to reproduce RBCs bioconcave shape using variable surface tension on the interface between two fluids.
The assessment of characterization and feasibility of rocket engines scaled to millimeter size, arrayed onto wafers for the propulsion of small spacecrafts, is closely related to the right prediction of the thermal response of the [...]
This paper presents the first, high-fidelity numerical study of nanofluidic streaming potential-based sample preconcentration. Parametric analysis is performed to capture the critical effects of pressure and background electrolyte (BGE) concentration on preconcentrator performance. Our studies [...]
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2008: Microsystems, Photonics, Sensors, Fluidics, Modeling, and Simulation – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: June 1, 2008
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Micro & Bio Fluidics, Lab-on-Chip