Rastogi S.K. – TechConnect Briefs

Silica coated Magnetic Nanoparticles (SMNPs): Capture and Identification of Escherichia coli Cells using Surface Enhanced Raman Spectroscopy

Rastogi S.K., Jabal J., Zhang H., Haler K.J., Gibson C.M., Qiang Y., Aston D.E., Hurdlicka P.J., Branen A.L., University of Idaho, US

Silica coated Magnetic nanoparticles (SMNPs; SiO2/Fe3O4 + Fe2O3) were prepared by encapsulation of iron oxide NPs with silica dioxide using the sol-gel method and characterized through transmission and scanning electron microscopic imaging, magnetic hysteresis and [...]

One Pot vs Two Steps Synthesis of Fluorescent Gold Nanoparticles and Capping with Thiolated Organic Linker

Rastogi S.K., Denn B.D., Branen A.L., University of Idaho, US

Nobel metal nanoparticles (NPs) such as Au and Ag have gained great attention in the past decades due to their fundamental optical properties. Based on that interest, the current work is focused for the synthesis [...]

Fluorescence Based Specific PNA-DNA Binding Characterization on nano-FET DNA Device Surface

Rastogi S.K., Mishra N.N., Finaloski B., Cameron E., Maki W., Postdoc Researcher, US

A novel sequence of peptide nucleic acid (PNA; beta-Ala-(T10)-beta-Ala-NH2) containing ten thymine monomers and beta-alanine amino acid at both (C and N) terminal of main chain was synthesized manually using solid phase tBoc peptide chemistry. [...]

Water Soluble Peroxyoamid Reagent: Evalulation for Fluorescent Silica Nano-Particles Chemilumineacence

Rastogi S.K., Fianaloski B., Mishra N.N., Cameron E., Bitterwolf T.E., Branen A.L., Maki W.C., University of Idaho, US

Chemiluminescence methods, both enzymatic and non-enzymatic, are widely used as an analytical tool in various bio-molecular detection applications. Advantages of using chemiluminscence detection are its practical simplicity, relatively low back ground interference, high sensitivity compared [...]

Fluorescent Silica Nano-Particles and Magnetic Bead Based Immunoassay: an Optical Tool for Detection of Staphylococcal enterotoxin B in Milk for High-Throughput Screening

Rastogi S.K., Branen J.R., Gibbon S., Larry Branen A., University of Idaho, US

This paper describes the use of fluorescent silica nanoparticles (FSNPs) as luminescent signal amplifiers in biological system based on digital counting of individual particles and measuring of average fluorescence intensity, because FSNPs emit intense yellow-green [...]

Amperometric Detection of Staphylococcal Enterotoxin B in milk using Magnetic Bead-Based Immunoassay on Disposable Screen Printed Carbon Electrode Strip

Rastogi S.K., Gibbon S., Branen A.L., Branen J.R., Branen A.L., Branen J.R., University of Idaho, US

A novel approach for the sensitive detection of Staphylococcal enterotoxin B (SEB) in milk is reported. The method used sandwich enzyme-linked immunoassay with SEB specific antibodies on magnetic beads. A small cylindrical magnet was fixed, [...]

Polysilicon Nano-wires Based Nano-device on Silicon Chip: Fabrication and its Application

Mishra N.N., Winterrowd P., Nelson R., Maki G., Maki W.C., Rastogi S.K., Cameron E., Filanoski B., Maki G., Maki W.C., University of Idaho, US

5x5 arrays of six nano-transistor devices were fabricated on p-type silicon/silicon oxide (200nm) wafers with resistivity 1-100 -cm. 50nm of doped polysilicon were deposited and annealed to make nanowires. All nanowires were made with direct [...]

Ultra-sensitive Electrochemical Detection of E. coli Using Nano-porous Alumina Membrane

Mishra N.N., Maki G., Maki W.C., Filanoski B., Fellegy M., Cameron E., Rastogi S.K., Maki G., Maki W.C., University of Idaho, US

A combinational method has been developed for ultra-sensitive detection of E. coli. The method combined biological, nano-fabrication and electrochemical techniques to achieve highly specific and ultra-sensitive detection of bacterial pathogen. E. coli K12 was used [...]

Polysilicon Nano-wires Based Nano-device on Silicon Chip: Fabrication and its Application

Mishra N.N., Winterrowd P., Nelson R., Maki G., Maki W.C., Rastogi S.K., Cameron E., Filanoski B., Maki G., Maki W.C., University of Idaho, US

5x5 arrays of six nano-transistor devices were fabricated on p-type silicon/silicon oxide (200nm) wafers with resistivity 1-100 -cm. 50nm of doped polysilicon were deposited and annealed to make nanowires. All nanowires were made with direct [...]

Functionalized Mesoporous Silica Nano-particle for Optical Bio-detection

Maki G., Maki W.C., Mishra N.N., Filanoski B., Rastogi S.K., Cameron E., Maki G., Maki W.C., University of Idaho, US

Mesoporous silica nano-particles were immobilized with luciferase and antibodies for immune-bioluminescence detection of Staphylocuccus aureus enterotoxin B (SEB) and C (SEC). Mesoporous silica nano-particles were used as matrix to immobilize antibodies specific for enterotoxins and [...]