University of Idaho – TechConnect Briefs

Silica coated Magnetic Nanoparticles (SMNPs): Capture and Identification of Escherichia coli Cells using Surface Enhanced Raman Spectroscopy

Rastogi S.K., Jabal J., Zhang H., Haler K.J., Gibson C.M., Qiang Y., Aston D.E., Hurdlicka P.J., Branen A.L., University of Idaho, US

Silica coated Magnetic nanoparticles (SMNPs; SiO2/Fe3O4 + Fe2O3) were prepared by encapsulation of iron oxide NPs with silica dioxide using the sol-gel method and characterized through transmission and scanning electron microscopic imaging, magnetic hysteresis and [...]

One Pot vs Two Steps Synthesis of Fluorescent Gold Nanoparticles and Capping with Thiolated Organic Linker

Rastogi S.K., Denn B.D., Branen A.L., University of Idaho, US

Nobel metal nanoparticles (NPs) such as Au and Ag have gained great attention in the past decades due to their fundamental optical properties. Based on that interest, the current work is focused for the synthesis [...]

Anti-K-Ras siRNA to Treat Pancreatic Cancer

Julien D.C., Giri A., Papasani M., Murdoch G., Hrdlicka P., Hill R.A., University of Idaho, US

Cancer of the pancreas is a leading cause of cancer-related mortality. Chemotherapeutic drugs used to treat pancreatic cancers are toxic and minimally effective, yielding a 1-4% 5-year survival rate after initial diagnosis. Approximately 90% of [...]

Transformation of gold nanoparticles into effective gene silencing vectors by oligoethylene glycol passivation

Papasani M.R., Pokharel D., Giri A., Sai V.V.R., Hrdlicka P.J., Hill R.A., University of Idaho, US

Small interfering RNAs (siRNAs) are useful agents for gene knockdown. We have taken a systematic approach to investigate the use of GNP as delivery platforms for firefly luciferase (FFL) siRNAs and the effect of passivation [...]

Role of Serum Proteins in the Cellular Uptake of Gold-Peptide Nanoconjugates

Wang G., Papasani M.R., Hrdlicka P.J., Hill R.A., University of Idaho, US

Gold nanoparticles (Au NPs) have been widely investigated as promising nanocarriers for therapeutics and other biologically active molecules. In the present study, two different cysteine-terminated peptides were used to form nanoconjugates with Au NPs (20 [...]

Novel Nanoprobes to Detect mRNA in situ Directed Against Mouse Pyruvate Dehydrogenas

Cheguru P., Østergaard M., Papasani M.R., Sai V.V.R., Wengel J., Hrdlicka P.J., Hill R.A., University of Idaho, US

Novel nanoscale synthetic locked nucleic acid (LNA) probes incorporating molecular beacon (MB) technology were developed. These novel MBs contained 2’-N-(pyren-1-yl)carbonyl-2’-amino-LNA monomers incorporated in the loop region. Specificity and sensitivity of MB designed to target the [...]

Nanoparticle-coated nanosprings for diagnostic applications using SERS based detection

Sai V.V.R., University of Idaho, US

Significant enhancements in the SERS signals may be obtained with elongated arrangement of silver or gold nanoparticles. Silica nanosprings (NS) with high surface to volume ratio are promising candidate to yield such sensor materials. In [...]

Bio-Molecules Immobilization on Nanowire Electronic Devices

Maki G., Maki W., Mishra N., Fellegy M., Cameron E., Maki G., Maki W., University of Idaho, US

Nano-scale Field Effect Transistor based devices (nano-FETs) have shown great promise in bio-molecular detection. The advantages of using electronic nano-devices verses using conventional bio-detection methods are: direct detection without labeling, ultra-sensitivity without amplification and simple [...]

Water Soluble Peroxyoamid Reagent: Evalulation for Fluorescent Silica Nano-Particles Chemilumineacence

Rastogi S.K., Fianaloski B., Mishra N.N., Cameron E., Bitterwolf T.E., Branen A.L., Maki W.C., University of Idaho, US

Chemiluminescence methods, both enzymatic and non-enzymatic, are widely used as an analytical tool in various bio-molecular detection applications. Advantages of using chemiluminscence detection are its practical simplicity, relatively low back ground interference, high sensitivity compared [...]

Fluorescent Silica Nano-Particles and Magnetic Bead Based Immunoassay: an Optical Tool for Detection of Staphylococcal enterotoxin B in Milk for High-Throughput Screening

Rastogi S.K., Branen J.R., Gibbon S., Larry Branen A., University of Idaho, US

This paper describes the use of fluorescent silica nanoparticles (FSNPs) as luminescent signal amplifiers in biological system based on digital counting of individual particles and measuring of average fluorescence intensity, because FSNPs emit intense yellow-green [...]