Direct Current Dielectrophoretic Characterization of Erythrocytes: Positive ABO blood types

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This work adapts DC-DEP to a medical diagnostic application of sorting blood cells. Initial efforts focus on achieving separation and collection of red blood cells based on their positive ABO blood types. A significant separation was observed for A+, B+, AB+, and O+ red blood cells when exposed to AC dielectrophoresis [3, 4]. These results obtained by the authors suggest that spatial separation in the field depends on the blood type antigen expressed on the surface of the red blood cells, which forms the basis of the present work. In DC-DEP, successful separation of cells into bins is dependent on the deflection from the insulating obstacle (Figure 1). Y. Kang etal. (2007) [1] showed that the DC-DEP force acting on the cell is proportional to the size of the cell. Therefore, two dependencies will simultaneously be explored: blood type and blood cell size. This is possible because blood type antigens are expressed only on the red blood cells (erythrocytes) while whole blood is comprised of three different cells ranging in size from XX to YY. Fluorescent polystyrene particles of three different sizes roughly corresponding to erythrocytes, leukocytes and platelets will be tested as a size dependence reference and compared against the separation and collection of actual blood cells into different bins. Further, continuous separation of red blood cells according to blood types and collection into specific bins will be explored. This developed technique is directly applicable for use in a portable device for easy and rapid blood diagnostics.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2008: Microsystems, Photonics, Sensors, Fluidics, Modeling, and Simulation – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: June 1, 2008
Pages: 401 - 404
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 978-1-4200-8505-1