GRAVI-Chip: Automation of Microfluics Affinity Assay based on Magnetic Nanoparticle

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An automated nanoparticle based microfluidic platform called GRAVI™ will be presented here as a novel benchtop robotised system for fast and automated ELISA analysis in low volumes. This system can handle samples and reagents from conventional plates or tubes and automatically pipette them in a tilted linear microchannel array (GRAVI™-Chip) arranged in a SBS compatible format. The sample, washing, conjugate and substrate solutions are pipetted successively in the upper ends of the microchannels and flow by gravity-induced pressure towards the bottom waste reservoirs. The affinity capture is ensured by functionalised nanomagnetic beads stopped with a programmable magnet addressing each microchannel individually. Detection is performed in parallel by electrochemical means, and the chips are regenerated after each assay either with an affinity elution buffer or by releasing the magnetic beads. As will be shown in this presentation, this novel platform provides an all-in-one solution replacing standard incubation, washing and detection stations, and facilitating handling and reducing instrumentation infrastructure, while speeding up bioanalytics thanks to the use of nanoparticles. The performances of this GRAVI™ microchip platform will also be presented, along with applications in the field of recombinant protein screening, where minimum manual handling is required and where short time-to-result is expected in order to monitor production process and for quality control purposes.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2008: Microsystems, Photonics, Sensors, Fluidics, Modeling, and Simulation – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: June 1, 2008
Pages: 231 - 233
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 978-1-4200-8505-1