Papers:
DNA Detection Using Metal-Fluorescence Interactions on Two-Dimensionally Assembled Gold Nanoparticles
We developed a novel DNA detection system using a single stranded probe oligonucleotide with a fluorophore immobilized on two-dimensionally assembled gold nanoparticles. This system enables the detection of amplified DNAs without fluorescence labeling. In the [...]
Rapid Isothermal Amplification and Multiplexed Surface Detection of Short DNA Sequences
We have coupled a novel isothermal amplification method for short oligonucleotides (EXPAR) with solution and surface-based detection involving DNA-functionalized gold nanospheres. In these assays, a trigger oligonucleotide is exponentially amplified and converted to a reporter [...]
Peptide Nucleic Acids Modified Nano-Biosensor for Early Cancer Diagnosis
Epigenetics is proving to have an increasingly important role in both human development and disease, particularly cancer. DNA methylation is one of two epigenetic alterations. A distinct set of tumor types has a high level [...]
Nanoplex(TM) Biotags: Near-IR Excited, Highly Multiplexed Nanoparticulate Optical Detection Tags for Diagnostic Assays
Oxonica Inc has developed a novel optical detection tag for use in point of care diagnostics, cellular imaging assays, and life science assays. Nanoplex(TM) Biotags are silica coated Au nanoparticles that, by virtue of surface [...]
Homogeneous Bioluminescence Resonant Energy Transfer On High Density Array Imaging Chip
Fluorescent microarray technologies are widely used in molecular biology for studying gene expression, immune detection and drug discovery. Fluorescent assays are very sensitive but present problems with many bio-agents having inherent fluorescence which interfere with [...]
DNA-DNA Interaction on Dendron-Functionalized Sol-Gel Silica Films Followed with Surface Plasmon Fluorescence Spectroscopy
Kwon S.H., Hong B.J., Park H.Y., Park J.W., Knoll W., Park H.Y., Park J.W., Center for Integrated Molecular System., KR
Since we observed that dendron-assembled surface providing a proper spacing between immobilized DNAs showed high single nucleotide polymorphism discrimination efficiency for DNA microarrays(1), the nanoscale-controlled surface is examined with surface plasmon field-enhanced fluorescence spectroscopy (or [...]
Evaluation of silver nanostructures fabricated using glancing angle depostion as localized surface plasmon resonance biosensors
The localized surface plasmon resonance (LSPR) biosensing capability of 175 nm thick silver nanoparticle films produced by glancing angle deposition (GLAD) was evaluated. The extinction spectra of the films exhibited a peak around a wavelength [...]
Fabrication and Characterization of Nano-Field Effect Transistor for Bio-safety
Mishra N., Winterrowd P., Nelson R., Rastogi S., Maki G., Maki W., Maki G., Maki W., University of Idaho, US
The sensitive and robust detection of harmful biological and chemical molecules in the various samples have incredible significance in modern healthcare. New research in the field of nanowires has evidence that nanowire based diagnostic methods [...]
Nanomechanical Cantilever Bio-Sensors for Time-Resolved Detection of DNA and Surface Layer Formation
Nanomechanical cantilever are small and extremely sensitive force and mass detectors. By coating (functionalizing) their surface with specific receptor molecules, cantilevers can be converted into highly sensitive and selective chemical sensors or label-free biosensors. The [...]
An amperometric on-chip glucose biosensor based on enzyme entrapment with pre-reaction to lower interference in a flow injection system
In this study, the flow injection analysis was applied to the enzyme-entrapped sensor on a chip for sensing glucose. The microelectrode arrays on a chip of 7×3.5 cm2 were fabricated by standard photolithographic and the [...]
Fabrication of Molecularly Imprinted Uric Acid Biosensors based on a Novel Amine-Imide Type Conducting Polymer
In this work, the major contribution is fabricating a uric acid (UA) biosensor based on molecular imprinted technique using a novel conducting polymer. Calibration curves were depicted according to the amperometric detection of steady current [...]
Nanoelectrodes with Selective Molecular Receptors for Label-Free Electronic Detection of Thrombin
The enzyme thrombin plays a crucial role in the regulation of blood coagulation. We present a sensor that can directly detect this enzyme and generate an electronic signal. The sensor structure consists of three electrodes [...]
Synthesis of a nano electro-magnetic polymer and its application in a DCT biosensor
This experimental study was focused on the development of a nanomagnetic conductive polymer and its application in a direct-charge transfer biosensor (Figure1) for the detection of Bacillus species. A polyaniline-γFe2O3 nanocomposite transducer was first synthesized [...]
Electronic DNA detection on semiconductor surfaces
Electronic biosensors offer a viable alternative to optical biosensors for the rapid, specific, and sensitive detection of DNA. We are utilizing an electrolyte-oxide-semiconductor (EOS) biosensor system on a SiO2/Si surface for impedimetric detection of DNA. [...]
Label-Free Protein Nano-Biosensor Using Top-Down Fabrication
Ah C.S., Ahn C-G, Kim A., Yu H.Y., Ahn C-G, Yang J.H., Baek I-B, Park C.W., Jun M.S., Lee S.J., Electronics and Telecommunications Research Institute (ETRI), KR
A nanosensor of n-type Si field-effect transistor (FET) via “top-down method” was employed to monitor PSA with great selectivity and high sensitivity up to 10 pg/mL. When a biomaterial, negatively charged PSA, is introduced through [...]
Development of micro-lateral flow strips compatible with micro-volume samples for immunochemical assays
The benefits of the lateral flow strip immunochromatographic test format are well known. However, currently available lateral flow strip assays are not compatible with sub-microliter sample volumes. The authors believe that assays compatible with micro-volume [...]
Specific and Non-specific adsorption of Proteins on Solid Interfaces
We present a comprehensive mechanistic study of the non-specific adsorption of β-Lactoglobulin on a solid interface by comparing the adsorption kinetics of this protein to theoretical predictions of reported models. Important experimental observations were increasing [...]
nanometric thinfilm sensor
We want to present an optical thin film sensor chip able to detect the decay of food through a specific color change. The design of the chip relates to a phenomenon, the so called “anomalous [...]
Biosensor for heavy metals using hydrothermally grown ZnO nanorod/nanotube and metal-binding peptides
Certain heavy metals can pose potential health risks when consumed by humans due to their high toxicity. Specifically, the heavy metals mercury, lead and cadmium can be released into the environment through industrial use and [...]
Stable Self-Assembled Monolayers for sensitive biosensor interfaces
Jans H., Jans K., Jans H., Jans K., Bonroy K., Frederix F., Reekmans G., Maes G., Borghs G., IMEC, BE
One of the most important requirements for making advanced biosensors and biochips is the attachment of biological receptors onto a solid support. For this reason, it is important to construct a stable and well-characterized interface [...]
Simultaneous Detection of Salmonella typhimurium and Bacillus anthracis Spores Using Phage-Based Magnetoelastic Biosensors
Deliberate contamination of the food supply has become a concern, Salmonella typhimurium and Bacillus anthracis spores being primary pathogens of interest. The objective of this research was to investigate simultaneous detection of these two biological [...]
A RC-based biosystem for measuring anti-oxidizing power
The goal of this work is the development of specific and sensitive biosensors for the measure of anti-oxidizing agents power, by exploiting the property of Reaction Centres complexes (RCs) to specifically recognize oxidizing chemicals (e.g. [...]
A Non-Oxidative Sensor Based on a Self-Doped Polyaniline/Carbon Nanotube Composite for Highly Sensitive and Selective Detection of the Neurotransmitter Dopamine
The ability to measure the concentration of dopamine sensitively and selectively could potentially be used for molecular diagnosis of Parkinson’s disease. The ability to monitor its concentration in-vivo also benefits in design therapies and evaluating [...]
Quantitative measurements of the strength of adhesion of human neutrophils to a substratum in a microfludic device: A novel inflammation diagnostic tool
We describe a quantitative assay of the strength of adhesion of activated and non-activated human neutrophils to a substratum, which is carried out in a custom-made microfluidic device. The strength of adhesion is quantified by [...]
Prevention and Risk Reduction in Heart Health
Identifying early onset of cardiac disease is not easy and it is very expensive. For this reason the first symptom of cardiac problems is often catastrophic. It is the goal to produce a low cost [...]
Encapsulation of Streptavidin labeled with Alexa Fluor 750 into Erythrocytes
Erythrocytes, Red Blood Cells (RBCs), have been utilized as biocompatible bioreactors and drug delivery vehicles. RBCs are also used as biological carriers for drugs, enzymes, and peptides. Hypo-osmotic dialysis technique is the most commonly employed [...]
Utilizing Silver and Gold Nanoparticles for Investigation of Bacterial Cell Wall Biochemical Structure
Understanding fundamental principles of living systems in their natural environment is always a challenge for scientists. The desire to design and construct novel diagnostic tools makes this understanding a significant prerequisite. Due to its “fingerprinting” [...]
Investigation of the Conductance of Silicon Nanowire Biosensors Using the 2D Drift-diffusion Model
DNA-FETs are DNA sensors consisting of a semiconductor transducer and a functionalized surface. Their device structure is similar to a MOSFET, but the gate has been replaced by the functionalized surface. Since DNA-FETs allow direct [...]
Parallel two channel near and far field fluorescence microscopy
We report a new two channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that [...]
Development of SERS Spectroscopy for Routine and Rapid Identification of Escherichia Coli and Listeria Monocytogenes on Ag Colloidal Nanoparticles
Rapid, accurate, and preferably routine methods for the identification of foodborne bacteria are increasingly important, because of bio- /agro-terrorism threats, public health concerns, and economic loss. Conventional detection of bacteria is time-consuming, requiring 5-7 days, [...]
Silver Particle and Silver Thiolate-Based Molecular Sensing/Recognition Units Operating via Surface-Enhanced Raman Scattering
Kim K., Kim N.H., Park H.K., Kim K., Kim N.H., Lee H.S., Lee Y.M., Lee H.S., Lee Y.M., Seoul National University, KR
Noble metallic nanostructures exhibit a phenomenon known as surface-enhanced Raman scattering (SERS) in which the scattering cross sections are dramatically enhanced for molecules adsorbed thereon. We demonstrate that commercially available Ag(μAg) powders can be used [...]
Single Molecule Interaction Forces and Recognition Imaging
Kienberger F., Chtcheglova L.A., Ebner A., Puntheeraunrak T., Gruber H.J., Hinterdorfer P., University of Linz, AT
In recent years, considerable attention has focused on biological applications of the atomic force microscope (AFM), in particular on high-resolution imaging of individual biological molecules and on the measurement of molecular forces under near-physiological conditions. [...]
Identifying Nanoscale Molecular Binding Events With SPM/AFM
PicoTRECTM is a chemical/biological detection accessory for the Agilent SPM/AFM (scanning probe microscopy/atomic force microscopy) that combines molecular scale imaging with nanoscale, molecular recognition mapping. It is being used to map interactions between antibodies/antigens and [...]
Quantitative Detection by Single Ion Channel Event Statistics: an Algorithmic Approach
The capability of having ligand-gated ion channels embedded in planar biomimetic membranes, together with a proper electronic system for elaborating the signal will give the possibility of creating high-performances sensors, able to detect low concentrations [...]
Using Spiral Inductors for Detecting Hybridization of DNAs Labeled with Magnetic Beads
This paper introduces an inductance-based sensor for detection of DNA hybridization and investigates its performance by means of computer simulation. In order to detect the occurrence of hybridization, single strand target DNA’s are tagged with [...]
Multiplexed immunoassays by flow cytometry for detection of Clenbuterol, Chloramphenicol and sulfadimidine with high sensitivity and selectivity
An accurate, rapid and cost-effective detection on the environmental hazardous chemicals is the cornerstone of efficient food safe management. Hereby we discuss the relevance of an emerging technology, multiplexed competitive immunoassays read by flow cytometry, [...]
Quantum Dots as Replacements for Tandem Dyes in Flow Cytometry
Qdot® nanocrystals provide fluorescent labels that can be excited with UV or violet light sources, but can also be used with longer wavelength excitation. In flow cytometry, investigators achieve greater multiplexing of cellular markers by [...]
Modified Nanoparticle Films for Transmission Plasmon Biosensing
Bonroy K., Frederix F., Reekmans G., Jans H., Jans K., Jans H., Jans K., Van de Broek B., De Palma R., Borghs G., IMEC, BE
The principle of the Transmission Plasmon Biosensor is based on LSPR of metal nanoparticles attached to a transparent substrate. When the analyte of interest binds to the bioreceptors immobilized onto these nanoparticles, the absorption properties [...]
Qdots as smart pH probes monitor proton flux driven by ATP synthesis and optical coder for detection various virus
The chromatophores allow self assemble with the negatively charged Qdots through electrostatic reaction. The fluorescence intensity of Qdots not only was vastly different in the solution and on the surface of chromatophores, but also for [...]
Nitrosyl-iron Complexes as Potent Smart Nitric Oxide Biosenors: MRI imaging Techniques in Progress
Nitric oxide is a gaseous, free radical which plays a role as a diffusible intracellular second messenger. It is emerging as biosensor for monitoring myocardial ischemia and the effect of HMG-CoA reductase inhibitor statins. Presently, [...]
Qdots as smart pH probes monitor proton flux driven by ATP synthesis and optical coder for detection various virus
Here we described a novel approach of CdTe quantum dots fluorescence probes to improve the detection of proton flux through F1F0-ATP by ATP synthesis. It was found that both the CdTe quantum dots (535nm, 585nm) [...]
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Industry sector: Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 1-4200-6183-6