Development of micro-lateral flow strips compatible with micro-volume samples for immunochemical assays

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The benefits of the lateral flow strip immunochromatographic test format are well known. However, currently available lateral flow strip assays are not compatible with sub-microliter sample volumes. The authors believe that assays compatible with micro-volume samples will be required to achieve market acceptance of general, routine and frequent, home diagnostics. MicroPlumbers Microsciences LLC has developed an inexpensive immunochemical marker screening and monitoring device that can operate with sample volumes of less than 1 microliter (Figure 1). The device relies on micro-miniaturized lateral flow strip technology to provide a reliable platform for sandwich and competitive immunoassays. The applicability of the method has been demonstrated with various commercially-available assay chemistries. The device is also of benefit to clinicians for rapid testing for panels of analytes, or researchers wishing to define their own panel of analytes. In this paper we will demonstrate the use of miniaturized lateral flow strips with various assays. A micro-lateral flow strip based device that can operate with sample volumes of less than 1 microliter has been demonstrated, as well as a parallel configuration that performs 10 independent assays from a single 25 microliter drop of sample.

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Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 496 - 499
Industry sector: Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 1-4200-6183-6