In this study, the flow injection analysis was applied to the enzyme-entrapped sensor on a chip for sensing glucose. The microelectrode arrays on a chip of 7×3.5 cm2 were fabricated by standard photolithographic and the microfluidic channel height of 100 micrometer was formed by combining the cover of poly(dimethylsiloxane). The conducting polymer, poly(3,4-ethylenedioxythiophene), was electropolymerized with glucose oxidase at a flow rate of 5 ml/hr on “WE1”. While in the background buffer, the enzyme electrode was applied a potential of 0.7 V (vs. Ag) by injecting different concentrations of glucose (Glu) sample for sensing H2O2. The linear regression from 0.1 mM to 20 mM with a sensitivity of 8 nA mm-2 mM-1. Besides, the response time (t95) and the recovery time are about 30 s and 230 s, respectively. The pre-reaction section, “WE2”, was applied at 0.7 V (vs. Ag) to electrocatalyze the oxidized-favor materials of the injecting samples. For the single-potential, the 0.08 mM AA and blend 0.08 mM AA and 10 mM Glu samples reached 31.3 % and 145.5 % of the sensing current of 10 mM Glu, respectively. In contrast, the sensing current of blend sample reached 99.6 % of 10 mM Glu in the bi-potential test.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 473 - 476
Industry sector: Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors