Fabrication of Molecularly Imprinted Uric Acid Biosensors based on a Novel Amine-Imide Type Conducting Polymer


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In this work, the major contribution is fabricating a uric acid (UA) biosensor based on molecular imprinted technique using a novel conducting polymer. Calibration curves were depicted according to the amperometric detection of steady current in different concentrations of UA (from 0 to 1.125 mM). The sensitivities of molecularly imprinted polymer (MIP) and non-molecularly imprinted polymer (NMIP) electrodes were calculated to 22.59 and 9.67 μA mM-1cm-2, respectively. Imprinting efficiency, defined as the ratio of sensitivity of MIP to that of NMIP electrode, was 2.3 in this work. The limit of detection (LOD) for the MIP-ITO/Poly(PD-BCD)UA electrode was calculated to be 3×10-4 mM on the basis of signal to noise ratio (S/N) equaling to 3. It was found that the MIP sensor showed relatively good sensitivity, large linear range, and high imprinting efficiency with a low LOD for sensing UA. The MIP-ITO/Poly(PD-BCD)UA was also tested for ascorbic acid (AA), a major interferent, under its normal concentration range in human plasma. The current response of 0.04 mM AA is less than 7 % of the current response of 0.4 mM UA. This shows that AA has a low interference for the MIP-ITO/Poly(PD-BCD)UA electrode in sensing UA.

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Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 477 - 480
Industry sector: Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 1-4200-6183-6