Oligonucleotides (ONs) such as antisense (ASO) or siRNA have created a need for new technologies that further enable the systemic and targeted delivery of these active principles. Here, we describe amphoteric, fully charge-reversible liposomes as [...]
RNA interference (RNAi) is a new, promising approach to selectively regulate gene expression through modulating mRNA stability and translation. However, the efficient delivery of 21 mer dsRNA molecules (siRNA) to affect RNAi remains a challenge. [...]
Calando Pharmaceuticals is developing siRNA-containing nanoparticle formulations, and its leading formulation that will enter the clinic in 2008 contains the human transferrin protein as a targeting ligand and siRNA targeting the M2 subunit of ribonucleotide [...]
As siRNA mediated therapy further develops, the need for biocompatible, effective delivery vectors, especially for mucosal delivery, is apparent. We report here a novel nanocarrier for siRNA delivery via targeted chemical modification of the polysaccharide [...]
We report a novel approach to efficiently package and deliver siRNAs with low generation dendrimers by using Au nanoparticles as a “labile catalytic” packaging agent. The Au nanoparticles helped low generation dendrimers to package siRNA [...]
There is an increasing enthusiasm for developing therapies based on RNA interference (RNAi). However, the main obstacle in siRNA therapy is RNA delivering to the cytoplasm where it can guide sequence-specific mRNA degradation. An efficient, [...]
RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA [...]
RNAi therapeutics represent a method to treat human diseases by addressing targets that are not possible to be addressed with conventional medicines. In our Centre we design nanosized systems for siRNA delivery that consist of [...]
To fully exploit the vast potential of RNA interference, the development of safe and efficient reagent to deliver small amount of siRNA is necessary. We have explored the potential of in vivo-jetPEI™, an optimized linear [...]
There is an increased interest in developing alternative therapies to combat deadly infectious disease including HIV. This work investigates the fundamentals of a novel, nanoparticle-based potential treatment method employing macrophage cells. Macrophages are a very [...]
Non-viral gene delivery and transfection in primary human vascular endothelial cells (EC) and smooth muscle cells (SMC) has tremendous potential for cardiovascular diseases, such as in treatment of coronary restenosis. Using a combination of synthetic [...]
We are exploring peptide nucleic acid (PNA) assemblies as interesting scaffolds for the fabrication of nanomaterials relevant to the diagnosis and treatment of disease. The first assembly is a PNA/LNA (locked nucleic acid) duplex employed [...]
, Belen Rubio M.
, Filpula D.
, Greenberger L.M.
, Horak I.D.
, Reddy P.
, Sapra P.
, Wang M.
, Wu D.
, Xia J.
, Zhao H.
, Zhu P.
, Enzon Pharmaceuticals, Inc., US
Delivery of LNA oligonucleotides using Customized Targeted PEG linkers has improved the in vitro cellular uptake into cancer cells. Customized Targeted PEG linker-LNA conjugates can knock down target mRNA in a concentration dependent fashion with [...]
In the past several years a lot of efforts have been made in the production of new high performance drugs without toxic and other side effects. First promising results were obtained using micro and nanoparticles [...]
The primary objective of this study is to evaluate novel cationic and degradable beta-cyclodextrin nanoparticles as drug delivery carriers for insulin across the bovine brain microvessel endothelial cell (BBMVEC) monolayer, an in vitro blood brain [...]
Dynamic observations have driven the course of imaging biology ever since the first bacterial motility was observed in the late sixteenth century. Continuous imaging of time dependent bacterial motion events is a rapidly emerging biomedical [...]
The objective of this work was to determine the influence of pH, water-soluble polymers and surfactants on mebendazole solubilization by beta- and permethyl-beta-cyclodextrins (beta-CD and PM-beta-CD). Mebendazole is an antihelmintic active drug used against a [...]
Summary An improved method of drug delivery is much needed in areas such as cancer therapy in which more drugs needs to be delivered to a specific site and non-specific toxicities should be minimal. Drug [...]
Through the considerate organisation of surface ligands, we have developed a lipid-based vector for the targeted delivery to cancer cells. Our ligand is a short peptide with high affinity for the Urokinase Plasminogen Activator receptor [...]
99mTc(V)DMSA (Dimercaptosuccinic acid) is a well-known radiopharmaceutical for imaging neurogenic tumors. 188Rhenium, a beta radiation emitter with 2.2 MeV energy having half life of 16.9 hours is a therapeutic analogue of imagable 99mTechnetium was introduced [...]
, Fang Z.
, He Q.
, Wang M.
, Wu Jian
, Xu Q.W.
, Yaun W.M.
, Zhang X.Z.
, Zhau D.M.
, Morehouse School of Medicine, US
Glaucoma, the leading cause of irreversible blindness in the world, is estimated to be affected by 67 million people. It is characterized by an increase in intraocular pressure (IOP). Carbonic anhydrase inhibitors (CAIs) are orally [...]
Although claims of improved performance due to nanoscale phenomena are made on a daily basis, exactly how much advantage can be afforded by nanotechnology for specific medical applications is quantitatively unknown. It is generally suggested [...]
The aim of this work was to optimize the preparation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with paromomycin by a nanoprecipitation technique. PLGA was choosen because of its biodegradability and biocompatibility features. In order to [...]
Mebendazole (MBZ) is an antihelmintic which belongs to group of benzimidazols. It is used for many intestinal diseases and for other diseases. But its low absorption, its high dosages caused side effects on gastro-intestinal track, [...]
Current concepts in the synthesis of nanocarriers primarily involve the use of polymers or lipids to fabricate self-assembled or emulsion-based particles that are mostly spherical, polydisperse, and release drugs through diffusion or hydrolysis. Although significant [...]
DNA-associated single walled carbon nanotubes(DNA:SWCNTs) have shown potential in drug delivery with the ability to translocate across the cell membrane carrying various biomolecules. Our laboratory has completed a series of experiments to show the sonication [...]
Defective folding diseases have emerged in the last decade as prevalent in an ageing population. In this respect any increased knowledge of folding mechanisms could be crucial for preventing, treating or even curing these neurodegenerative [...]
, Cheng A.
, Li M.
, Liang Y.
, Magdaleno S.
, Vlassov A.
, Wang Y.
, Wong L.
, Ambion Inc., An Applied Biosytems Business, US
We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be [...]
Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Industry sector: Medical & Biotech
Topicss: Biomaterials, Materials for Drug & Gene Delivery