SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers

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RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA antigens in a single gene carrier system. Our hypothesis is that, co-delivery of IL-10 specific SiRNA along with DNA antigens to the same dendritic cells would inhibit IL-10 production, enhance DC activation, antigen presentation and T cell response. Non-toxic, polyethyleneimine (PEI)-functionalized, poly(lactic-co-glycolic acid) (PEI-PLGA) microparticles co-delivering IL-10 SiRNA and DNA antigen exhibited significantly higher (p

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Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 350 - 353
Industry sector: Medical & Biotech
Topicss: Biomaterials, Materials for Drug & Gene Delivery
ISBN: 978-1-4200-8504-4