Papers:
Amphoteric liposomes are a platform for multi-organ delivery of oligonucleotides
Oligonucleotides (ONs) such as antisense (ASO) or siRNA have created a need for new technologies that further enable the systemic and targeted delivery of these active principles. Here, we describe amphoteric, fully charge-reversible liposomes as [...]
Oral Delivery of Map4K4 siRNA to Macrophages Inhibits TNF alpha Production and Protects against a LPS-Galactosamine Challenge in Mice
Aouadi M., Tesz G., Soto E., Wang M., Nicoloro S., Ostroff G., Czech M., University of Massachusetts Medical School, US
RNA interference (RNAi) is a new, promising approach to selectively regulate gene expression through modulating mRNA stability and translation. However, the efficient delivery of 21 mer dsRNA molecules (siRNA) to affect RNAi remains a challenge. [...]
Systemic Delivery of Targeted, siRNA-Containing Nanoparticles for the Treatment of Solid Tumors: Concept to Clinic
Calando Pharmaceuticals is developing siRNA-containing nanoparticle formulations, and its leading formulation that will enter the clinic in 2008 contains the human transferrin protein as a targeting ligand and siRNA targeting the M2 subunit of ribonucleotide [...]
Efficient siRNA Delivery by Secondary and Tertiary Amine Modified Polysaccharides
As siRNA mediated therapy further develops, the need for biocompatible, effective delivery vectors, especially for mucosal delivery, is apparent. We report here a novel nanocarrier for siRNA delivery via targeted chemical modification of the polysaccharide [...]
Labile Catalytic Packaging and Delivering of Short Interference RNA to Cancer Cells: Control of Gold Nanoparticles “out” of RNA Complexes
We report a novel approach to efficiently package and deliver siRNAs with low generation dendrimers by using Au nanoparticles as a “labile catalytic” packaging agent. The Au nanoparticles helped low generation dendrimers to package siRNA [...]
Toward in vivo Targeting Delivery of siRNA for Efficient Cancer Therapy
There is an increasing enthusiasm for developing therapies based on RNA interference (RNAi). However, the main obstacle in siRNA therapy is RNA delivering to the cytoplasm where it can guide sequence-specific mRNA degradation. An efficient, [...]
SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers
RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA [...]
Multilayered ABCD Nanoparticles for siRNA delivery
RNAi therapeutics represent a method to treat human diseases by addressing targets that are not possible to be addressed with conventional medicines. In our Centre we design nanosized systems for siRNA delivery that consist of [...]
Delivery systems for enhanced siRNA delivery
To fully exploit the vast potential of RNA interference, the development of safe and efficient reagent to deliver small amount of siRNA is necessary. We have explored the potential of in vivo-jetPEI™, an optimized linear [...]
Cell-mediated, heat-based therapy for combating HIV infection
There is an increased interest in developing alternative therapies to combat deadly infectious disease including HIV. This work investigates the fundamentals of a novel, nanoparticle-based potential treatment method employing macrophage cells. Macrophages are a very [...]
Gene Delivery and Transfection Studies with Lipopolyplexes in Human Endothelial and Smooth Muscle Cells
Non-viral gene delivery and transfection in primary human vascular endothelial cells (EC) and smooth muscle cells (SMC) has tremendous potential for cardiovascular diseases, such as in treatment of coronary restenosis. Using a combination of synthetic [...]
Development of Peptide Nucleic Acid Assemblies for Application in Drug Delivery and Molecular Sensing
We are exploring peptide nucleic acid (PNA) assemblies as interesting scaffolds for the fabrication of nanomaterials relevant to the diagnosis and treatment of disease. The first assembly is a PNA/LNA (locked nucleic acid) duplex employed [...]
Utility of Customized Targeted PEG Linkers for the delivery of oligonucleotides without the use of transfection reagents
Zhao H., Reddy P., Xia J., Wang M., Sapra P., Bandaru R., Belen Rubio M., Wu D., Zhu P., Filpula D., Greenberger L.M., Horak I.D., Enzon Pharmaceuticals, Inc., US
Delivery of LNA oligonucleotides using Customized Targeted PEG linkers has improved the in vitro cellular uptake into cancer cells. Customized Targeted PEG linker-LNA conjugates can knock down target mRNA in a concentration dependent fashion with [...]
Nanoparticles of the Thermo-mechanically Activated Zeolite in the Design and Synthesis of New Drugs Generation
In the past several years a lot of efforts have been made in the production of new high performance drugs without toxic and other side effects. First promising results were obtained using micro and nanoparticles [...]
Invention of Polysaccharide-base Nanoparticles for Enhancing Drug Permeability across the Blood Brain Barrier
The primary objective of this study is to evaluate novel cationic and degradable beta-cyclodextrin nanoparticles as drug delivery carriers for insulin across the bovine brain microvessel endothelial cell (BBMVEC) monolayer, an in vitro blood brain [...]
Molecular Imaging by Nanoparticle Bacterial delivery Systems:
Dynamic observations have driven the course of imaging biology ever since the first bacterial motility was observed in the late sixteenth century. Continuous imaging of time dependent bacterial motion events is a rapidly emerging biomedical [...]
Influence of water-soluble polymers and surfactants, on mebendazole solubilization by _- and permethyl-_-cyclodextrins
Ba O-M., Lahiani-Skiba M., Joudieh S., Bounoure F., Lahiani-Skiba M., Skiba M., Université de Rouen, FR
The objective of this work was to determine the influence of pH, water-soluble polymers and surfactants on mebendazole solubilization by beta- and permethyl-beta-cyclodextrins (beta-CD and PM-beta-CD). Mebendazole is an antihelmintic active drug used against a [...]
Synthesis of magnetic nanoparticles and its applications in drug delivery systems
Summary An improved method of drug delivery is much needed in areas such as cancer therapy in which more drugs needs to be delivered to a specific site and non-specific toxicities should be minimal. Drug [...]
Organisation of peptide ligands on the surface of gene vectors
Through the considerate organisation of surface ligands, we have developed a lipid-based vector for the targeted delivery to cancer cells. Our ligand is a short peptide with high affinity for the Urokinase Plasminogen Activator receptor [...]
Radiolabelled Microspheres As a Delivery System For Targetad Radiotherapy
99mTc(V)DMSA (Dimercaptosuccinic acid) is a well-known radiopharmaceutical for imaging neurogenic tumors. 188Rhenium, a beta radiation emitter with 2.2 MeV energy having half life of 16.9 hours is a therapeutic analogue of imagable 99mTechnetium was introduced [...]
Lyceum Chinense and Calcium Phosphate Nanoparticles for Ophthalmic Drug Delivery
He Q., Fang Z., Wang M., Xu Q.W., Chen R., Zhang X.Z., Yaun W.M., Zhau D.M., Wu Jian, Morehouse School of Medicine, US
Glaucoma, the leading cause of irreversible blindness in the world, is estimated to be affected by 67 million people. It is characterized by an increase in intraocular pressure (IOP). Carbonic anhydrase inhibitors (CAIs) are orally [...]
How much advantage do Nanoscale Phenomena afford for Pulmonary Delivery?
Although claims of improved performance due to nanoscale phenomena are made on a daily basis, exactly how much advantage can be afforded by nanotechnology for specific medical applications is quantitatively unknown. It is generally suggested [...]
Evaluation of the paromomycin loading characteristics in nanoprecipitated PLGA nanospheres
Kalimouttou S., Lahiani-Skiba M., Naouli N., LIN V.S., Lahiani-Skiba M., Skiba M., Université de Rouen, FR
The aim of this work was to optimize the preparation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with paromomycin by a nanoprecipitation technique. PLGA was choosen because of its biodegradability and biocompatibility features. In order to [...]
Preparation And Evaluation Of In Vitro Release Of Amphiphilic _-Cyclodextrin Or Pla Nanospheres Containing A Hydrophobic Drug
Mebendazole (MBZ) is an antihelmintic which belongs to group of benzimidazols. It is used for many intestinal diseases and for other diseases. But its low absorption, its high dosages caused side effects on gastro-intestinal track, [...]
Step and Flash Imprint Lithography for the Fabrication of Shape-specific, Enzymatically-triggered, Drug Nanocarriers
Current concepts in the synthesis of nanocarriers primarily involve the use of polymers or lipids to fabricate self-assembled or emulsion-based particles that are mostly spherical, polydisperse, and release drugs through diffusion or hydrolysis. Although significant [...]
DNA-Associated Single-walled Carbon Nanotubes as a Platform for Drug Delivery
DNA-associated single walled carbon nanotubes(DNA:SWCNTs) have shown potential in drug delivery with the ability to translocate across the cell membrane carrying various biomolecules. Our laboratory has completed a series of experiments to show the sonication [...]
Studies of conformational changes induced in a carrier protein: Bovine serum albumin
Defective folding diseases have emerged in the last decade as prevalent in an ageing population. In this respect any increased knowledge of folding mechanisms could be crucial for preventing, treating or even curing these neurodegenerative [...]
Real-time Detection and Quantitation of siRNAs in vivo
Cheng A., Liang Y., Vlassov A., Li M., Liang Y., Wang Y., Wong L., Chen C., Cheng A., Magdaleno S., Ambion Inc., An Applied Biosytems Business, US
We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be [...]
Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Industry sector: Medical & Biotech
Topics: Biomaterials, Materials for Drug & Gene Delivery
ISBN: 978-1-4200-8504-4