siRNA – TechConnect Briefs

HA-decorated nanoparticles as tumour-targeting nucleic acids carriers

Tirella A., Rios J.M., Tirelli N., University of Manchester, UK

The design of tools that allow for a targeted delivery of therapeutic cargos have a great potential in the treatment of many pathological conditions (e.g. cancer and autoimmune diseases). In the context of nucleic acids [...]

β3 Integrin siRNA-Loaded Multifunctional Nanoparticle Therapy for Metastatic Breast Cancer

Gujrati M., Parvani J.G., Schiemann W.P., Lu Z.R., Case Western Reserve University, US

Successful siRNA-mediated cancer therapy requires the use of multifunctional nanoparticle systems to overcome the barriers associated with delivery. We have recently developed a cationic lipid-based carrier, ECO, which efficiently delivers siRNA intracellularly to induce potent [...]

Cationic lipid nanoemulsions for RNAi screening

Bruniaux J., Sulpice E., Mittler F., Texier I., Gidrol X., Navarro F.P., CEA/LETI/MINATEC, FR

Since the discovery of RNA interference, many efforts have been made to broaden perspectives of small interfering RNA (siRNA) in research and clinical work. siRNA shows a specific and effective gene silencing activity through a [...]

Nanosystem for targeting Treg in vivo

Bottini M., Bottini N., Sacchetti C., Rapini N., Rosato N., Magrini A., Bottini M., Bottini N., Sanford Burnham Medical Research Institute, US

Due to their ability to suppress the activity of autoreactive cells, “natural” CD4+ CD25high FoxP3+ T cells (Treg) are important drug targets for autoimmune diseases and malignancies. Immunological studies are revealing a growing subset of [...]

Cationic Nanoparticles Effectively Delivered VEGF SiRNA in A549 Cells

Yang T., Li X., Liu Y., Liu Y., Bai S., Husson University, US

Recent discovery of small interfering RNA (SiRNA) that silences growth factors appears to improve effectiveness and safety of cancer therapy. Unfortunately, delivery of SiRNA to cancer cells is a major obstacle. Our work is focused [...]

siRNA and Paclitaxel Loaded PEG-PCL-PEI Tri-block Polymeric Micelle for Gene and Chemo-therapy Applications in Multidrug-resistance cancer cell

Hsu C.Y., National Chung Hsing University, TW

An annoying problem of chemotherapy was the drug resistance of cancer cell which was expression of multidrug-resistance associated proteins and resulted in ineffective treatment. To conquer this problem, a small interfering RNA (siRNA) molecule and [...]

siRNA Delivery Using Biodegradable Nanoparticles for Breast Cancer Therapy

Abbasi S., Arghya P., Afshan K., Prakash S., McGill University, CA

Polyethylenimine (PEI)-coated human serum albumin (HSA) nanoparticles form suitable biocompatible carriers for therapeutic molecules. Small interfering RNA (siRNA) molecules have suggested great promise as a future therapy for breast cancer. In order to overcome the [...]

Anti-K-Ras siRNA to Treat Pancreatic Cancer

Julien D.C., Giri A., Papasani M., Murdoch G., Hrdlicka P., Hill R.A., University of Idaho, US

Cancer of the pancreas is a leading cause of cancer-related mortality. Chemotherapeutic drugs used to treat pancreatic cancers are toxic and minimally effective, yielding a 1-4% 5-year survival rate after initial diagnosis. Approximately 90% of [...]

A proof of concept: a model of targeted stealth nanoparticles that are stable and biocompatible for RNA interference in vivo to macrophages

Yusuf M.S., Miller A.D., Prata C.H., Imperial College Gene Therapies Centre, UK

Bringing together advances generated through the developing fields of bionanotechnology and gene therapy, and demonstrated through the ABCD model, we propose a biocompatible, stable, specific stealth lipoplex targeting macrophages, as proof of concept, which is [...]

Multifunctional Polymeric Nanosystems for EGFR Gene Silencing

Pai S., Amiji M., Northeastern University, US

Multifunctional polymeric nanoparticles with an average size of 200nm could be prepared using the solvent displacement technique. Cetyltriethylammonium bromide (CTAB) surface-modified poly(ethylene oxide)-modified poly(epsilon-caprolactone) (PEO-PCL) nanoparticles encapsulated PTX and siRNA with a loading efficiency > [...]