siRNA – Page 2 – TechConnect Briefs

Delivery systems for enhanced siRNA delivery

Bolcato-Bellemin A-L., Bonnet M-E., Behr J-P., Erbacher P., Polyplus-transfection, FR

To fully exploit the vast potential of RNA interference, the development of safe and efficient reagent to deliver small amount of siRNA is necessary. We have explored the potential of in vivo-jetPEI™, an optimized linear [...]

Toward in vivo Targeting Delivery of siRNA for Efficient Cancer Therapy

Taratula O., Kirkpatrick P., Salva R., Pandya I., Minko T., He H., Rutgers-Newark University, US

There is an increasing enthusiasm for developing therapies based on RNA interference (RNAi). However, the main obstacle in siRNA therapy is RNA delivering to the cytoplasm where it can guide sequence-specific mRNA degradation. An efficient, [...]

Labile Catalytic Packaging and Delivering of Short Interference RNA to Cancer Cells: Control of Gold Nanoparticles “out” of RNA Complexes

Chen A.M., Taratula O., Wei D., Minko T., He H., Rutgers, The State of New Jersey, US

We report a novel approach to efficiently package and deliver siRNAs with low generation dendrimers by using Au nanoparticles as a “labile catalytic” packaging agent. The Au nanoparticles helped low generation dendrimers to package siRNA [...]

Systemic Delivery of Targeted, siRNA-Containing Nanoparticles for the Treatment of Solid Tumors: Concept to Clinic

Heidel J.D., Liu J.Y-C., Zeidan R.K., Liang Y., Rele S., Davis M.E., Calando Pharmaceuticals, US

Calando Pharmaceuticals is developing siRNA-containing nanoparticle formulations, and its leading formulation that will enter the clinic in 2008 contains the human transferrin protein as a targeting ligand and siRNA targeting the M2 subunit of ribonucleotide [...]

Amphoteric liposomes are a platform for multi-organ delivery of oligonucleotides

Reinsch C., Rauchhaus U., Panzner S., novosom AG, DE

Oligonucleotides (ONs) such as antisense (ASO) or siRNA have created a need for new technologies that further enable the systemic and targeted delivery of these active principles. Here, we describe amphoteric, fully charge-reversible liposomes as [...]

Multifunctional siRNA Delivery System for Cancer Therapy

Taratula O., Salva R., Pandya I., Wang A., Minko T., He H., Rutgers-Newark University, US

The main obstacle to siRNA therapy is in delivering RNA across the cell membrane. Furthermore, new methods for monitoring therapeutic responses are highly desirable. Here we report the development of a multifunctional nanomedicine platform for [...]

Sirna Nanomedicines for Cancer Gene Therapy

Kim S-H, Park T.G., Korea Advanced Institute of Science and Technology, KR

We developed a polyelectrolyte complex (PEC) micelle-based delivery system for treatment of prostate cancer. A PEGylated siRNA targeting human vascular endothelial growth factor (hVEGF) was synthesized. The hVEGF expression was almost completely inhibited in vitro [...]

Characterization and in vivo efficacy of targeted nanoparticles for systemic siRNA delivery to tumors

Bartlett D.W., Davis M.E., California Institute of Technology, US

This abstract highlights the results from our latest work in the design and application of targeted nanoparticles for systemic siRNA delivery. Over the past several years, we have performed extensive physicochemical and biological characterization of [...]