Medical & Biotech – Page 112 – TechConnect Briefs

SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers

Singh A., Nie H., Ghosn B., Roy K., The University of Texas at Austin, US

RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA [...]

Toward in vivo Targeting Delivery of siRNA for Efficient Cancer Therapy

Taratula O., Kirkpatrick P., Salva R., Pandya I., Minko T., He H., Rutgers-Newark University, US

There is an increasing enthusiasm for developing therapies based on RNA interference (RNAi). However, the main obstacle in siRNA therapy is RNA delivering to the cytoplasm where it can guide sequence-specific mRNA degradation. An efficient, [...]

Labile Catalytic Packaging and Delivering of Short Interference RNA to Cancer Cells: Control of Gold Nanoparticles “out” of RNA Complexes

Chen A.M., Taratula O., Wei D., Minko T., He H., Rutgers, The State of New Jersey, US

We report a novel approach to efficiently package and deliver siRNAs with low generation dendrimers by using Au nanoparticles as a “labile catalytic” packaging agent. The Au nanoparticles helped low generation dendrimers to package siRNA [...]

Efficient siRNA Delivery by Secondary and Tertiary Amine Modified Polysaccharides

Ghosn B., Singh A., Roy K., The University of Texas at Austin, US

As siRNA mediated therapy further develops, the need for biocompatible, effective delivery vectors, especially for mucosal delivery, is apparent. We report here a novel nanocarrier for siRNA delivery via targeted chemical modification of the polysaccharide [...]

Systemic Delivery of Targeted, siRNA-Containing Nanoparticles for the Treatment of Solid Tumors: Concept to Clinic

Heidel J.D., Liu J.Y-C., Zeidan R.K., Liang Y., Rele S., Davis M.E., Calando Pharmaceuticals, US

Calando Pharmaceuticals is developing siRNA-containing nanoparticle formulations, and its leading formulation that will enter the clinic in 2008 contains the human transferrin protein as a targeting ligand and siRNA targeting the M2 subunit of ribonucleotide [...]

Oral Delivery of Map4K4 siRNA to Macrophages Inhibits TNF alpha Production and Protects against a LPS-Galactosamine Challenge in Mice

Aouadi M., Tesz G., Soto E., Wang M., Nicoloro S., Ostroff G., Czech M., University of Massachusetts Medical School, US

RNA interference (RNAi) is a new, promising approach to selectively regulate gene expression through modulating mRNA stability and translation. However, the efficient delivery of 21 mer dsRNA molecules (siRNA) to affect RNAi remains a challenge. [...]

Amphoteric liposomes are a platform for multi-organ delivery of oligonucleotides

Reinsch C., Rauchhaus U., Panzner S., novosom AG, DE

Oligonucleotides (ONs) such as antisense (ASO) or siRNA have created a need for new technologies that further enable the systemic and targeted delivery of these active principles. Here, we describe amphoteric, fully charge-reversible liposomes as [...]

Drug & Gene Delivery Systems

Functional Polymersomes (Synthosomes) for Bioconversion and Selective Product Recovery

Güven A., Ihle S., Fioroni M., Schwaneberg U., Jacobs University Bremen, School of Engineering and Science, DE

The combination of natural proteins with polymer membranes allows the formation of a novel nanocompartment combining the mechanical, chemical, and biological stability of the amphiphilic block copolymer with the functional specificity of membrane proteins. Functional [...]

Biological influence of nanofibrous hydroxyapatite-polycaprolactone membrane on human periodontal ligament cell

Kim H.W., Kim T.I., Lee S.J., Ku Y., Rhyu I.C., Chung C.P., Han S.B., Kim H.W., Kim T.I., Seoul National University, KR

This study was performed to evaluate the effect of hydroxyapatite-coated nanofibrous polycaprolactone membrane on proliferation and differentiation of periodontal ligament cell which is necessary for periodontal regeneration. Hydroxyapatite-coated nanofibrous polycaprolactone membrane was fabricated through electrospinning [...]