Biological influence of nanofibrous hydroxyapatite-polycaprolactone membrane on human periodontal ligament cell

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This study was performed to evaluate the effect of hydroxyapatite-coated nanofibrous polycaprolactone membrane on proliferation and differentiation of periodontal ligament cell which is necessary for periodontal regeneration. Hydroxyapatite-coated nanofibrous polycaprolactone membrane was fabricated through electrospinning process and used as a test group. Polystyrene culture dish surface was regarded as a control group. Human periodontal ligament cells were seeded on each group and cultured until 14 days. Cell attachment morphology was observed by SEM. MTS assay and ALP assay were performed to determine cell proliferation and differentiation respectively. Mineral formation was examined by Alizarin red S staining. Statistical analysis was performed by Mann-Whitney U test with 95 % confidence interval. SEM revealed good human periodontal ligament fibroblast spreading on hydroxyapatite-coated polycaprolactone. MTS assay showed increased cell proliferation on hydroxyapatite-coated polycaprolactone membrane during 14 days. From 7 to 14 days, ALP activity of test group was significantly increased comparing to that of control group. Hydroxyapatite-coated polycaprolactone membrane showed abundant mineral formation at 14 day. Within the limitation of this study, we can conclude that hydroxyapatite-coated polycaprolactone membrane has favorable effects on proliferation and differentiation of human periodontal ligament cell and might be a candidate material for periodontal tissue regeneration.

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Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 322 - 324
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Biomaterials, Materials Characterization & Imaging
ISBN: 978-1-4200-8504-4