Papers – Page 57 – TechConnect Briefs

CNTs-Insulator- Semiconductor System for Chemical and Biological Sensor Applications

Cheon J-H, Lin Q., Kong B-D, Kang D.W., Kim L., Suh J.S., Park Y.J., Min H.S., Seoul National University, KR

In this paper, an Electrolyte-CNTs-Insulator-Semiconductor capacitor has been prepared due to compatibility with CMOS technology. To prepare this capacitor, we used the bonding technique to combine the MWNT array.It shows that the surface potential modulation [...]

Utilizing Nanoscale Patterned Proteins to Create Neuronal Cells Circuits

Wang W., Chun C-J, Gregory C., Narayanan S., Poeta M., Hickman J.J., University of Centra Florida, US

Micro-patterning of neuronal cells in vitro is a critical step for studies in the fundamental biology of neuron-neuron and neuron-surface interactions. The culturing of the neuronal cells on patterned self-assembled monolayers (SAMs), in some cases, [...]

Modeling Particle Hydrodynamic Transport in an Idealized Nanoscale Bio-Motor

Longest P.W., Pidaparti R.M., Virginia Commonwealth University, US

In the cell, hydrodynamic characteristics of the nuclear pore complex (NPC) are characterized by Stokesian liquid flow dynamics and high relative particle diffusion rates. As such, the hydrodynamics model of the NPC will be based [...]

Temperature Uniformity and DNA Amplification Efficiency in Micromachined Glass PCR Chip

Huang C-C, Wang M., Liu W-P, Weng K-Y, Yao L-Y, Industrial Technology Research Institute, TW

We have fabricated glass-based micromachined DNA polymerase chain reaction (PCR) chips with different heater patterns. Two pieces of Pyrex 7740 glass are thermally bonded together to form the PCR reaction channel. The PCR channel was [...]

Micropatterned Laminin-1 Matrices

Sgarbi N., Pisignano D., Di Benedetto F., Aloisi A., Nicolardi G., Cingolani R., Rinaldi R., Leece University, IT

We have obtained self-assembled laminin-1 networks on glass, realized by one-step microcontact printing lithography, without any preliminary functionalization of the substrates. We investigated the realized patterns by indirect immunofluorescence and atomic force microscopy (AFM), in [...]

Purification of PCR-Inhibitory Components by Cellulose Acetate Membranes

Mohamed H., Szarowski D.H., Lepak L.A., Spencer M.G., Martin D.L., Caggana M., Turner J.N., New York State Department of Health, US

Conventional methods for extracting DNA from blood results in the presence of heme, which is an inhibitory factor decreasing PCR sensitivity. Current techniques for removing the inhibitors are laborious, costly, and/or sample-specific. We investigated using [...]

Enhancement of E6 Protein Binding on Binding-Orientation-Sensitive Mixed SAMs Molecules

Tseng F-G, Tseng Y.T., Hwang J.K., Hung S.W., Chang J.M., Huang H.M., Huang T.W., Tseng F-G, Tseng Y.T., Huang H.M., Huang T.W., Chieng C-C, National Tsing Hua University, TW

Orientation selectivity and binding enhancement with E6 protein are demonstrated by nano-structured mixed-SAMs systems of different chain lengths. Mixtures of n-alkinethiol SAMs of different chain lengths: 1-Decanethiol (C9) and 1-Hexanethiol (C5) are the examples in [...]

Effect of Length of Biotin Modified n-Alkanethiolates on the Formation of a Self-Assembled Streptavidin-Biotin Monolayer on a Gold Surface

Kim D.-J., Cho E.C., Koo K.-K., Sogang University, KR

The molecular recognition between streptavidin and biotin makes the streptavidin-biotin system very useful in a wide range of biotechnological applications. Currently, biotin-modified n-alkanethiolates have been extensively studied to create a wide variety of self-assembled monolayers [...]

A Novel Electrochemical Nitric Oxide Sensor: Aligned RuO2 Nanowires Deposited on Pt filament

Wang C.Y., Liu J., Piantadosi C.A., Allen B.W., Duke University, US

We fabricated a novel electrochemical NO sensor by depositing aligned ruthenium oxide (RuO2) nanowires on a fine Pt filament. This device exhibited a high current density and a low detection limit for NO in aqueous [...]

Electrochemical Detection of Single-Nucleotide Mismatches: Application of M-DNA

Abu-Irhayem E., Long Y-T., Sutherland T., Li C-Z, Kraatz H-B, Lee J.S., University of Saskatchewan, CA

The detection of a single-nucleotide mismatch in a 20 base pair duplex DNA, using Electrochemical Impedance Spectroscopy, is presented. Mismatched DNA monolayers on Au electrodes were studied as normal duplex DNA (B-DNA) and after conversion [...]