immunoassay – TechConnect Briefs

Multiplex Express In Vitro Diagnostics based on Magnetic Nanoparticles

Nikitin M.P., Nikitin P.I., Guteneva N.V., Bragina V.A., Znoyko S.L., Orlov A.V., Gorshkov B.G., Nikitin M.P., Nikitin P.I., General Physics Institute, Russian Academy of Sciences, RU

Magnetic nanoparticles (MP) have provided many breakthrough solutions for life science. The immense potential of MP as labels in advanced immunoassays stems from the fact that they, unlike optical labels, can be easily detected inside [...]

Ultrasensitive and rapid immuno-detection of bacteria and protein biomarkers using field-effect enzymatic detection

Yau S.-T., Cleveland State University, US

A novel immuno-detection system has been constructed by incorporating the newly invented field-effect enzymatic detection (FEED) technique the immunosensing technique to demonstrate a novel detection platform. The demonstration consists of the detections of three biomarkers [...]

A double-bead sandwich assay for protein detection in serum based on localized surface plasmon resonance

Jans H., Jans K., Jans H., Jans K., Stakenborg T., Borghs G., Maes G., Lagae L., IMEC, BE

In this study, the importance of pre-concentration is clearly demonstrated for the covalent immobilization of various proteins to magnetic nanoparticles through mild carbodiimide activation. Additionally, the covalently biofunctionalized magnetic nanoparticles are employed in a double-bead [...]

Functionalized Nanopipette: towards single cell immunoassay

Actis P., Jejelowo O., Pourmand N., UC Santa Cruz, US

Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies [...]

Label-free direct detection of analytes from optically inaccessible samples

Brousseau L.C., Quantum Logic Devices, US

Fluorescent optical methods for biomolecular detection are widely used for research and diagnostic applications. However, these techniques are useful only in sample matrices that are optically transparent non-scattering, which precludes most biologically relevant fluids, such [...]

Intrinsic oxidase activity of cerium oxide nanoparticles facilitate the detection of cancer biomarkers and cancer cells

Perez J.M., Asati A., Santra S., Kaittanis C., Nath S., University of Central Florida, US

Nanomaterials exhibit unique size-dependent physical properties. Thus, identifying novel properties of these materials at the nanoscale, and their applications in energy, catalysis and medicine, among others, is an area of current and intense research. Cerium [...]

Water Soluble Peroxyoamid Reagent: Evalulation for Fluorescent Silica Nano-Particles Chemilumineacence

Rastogi S.K., Fianaloski B., Mishra N.N., Cameron E., Bitterwolf T.E., Branen A.L., Maki W.C., University of Idaho, US

Chemiluminescence methods, both enzymatic and non-enzymatic, are widely used as an analytical tool in various bio-molecular detection applications. Advantages of using chemiluminscence detection are its practical simplicity, relatively low back ground interference, high sensitivity compared [...]

Ferromagnetic Resonance Biochip for Diagnosing Pancratic Cancer

Casler E., Chae S., Kunduru V., Bothara M., Yang E., Ghionea S., Dhagat P., Prasad S., Portland State University, US

The goal of this research is to be able to develop a device that would be able to detect and diagnosis pancreatic cancer at bedside. The key criteria to be addressed are the cost, portability, [...]

‘Lab on a chip’ Label Free Protein Sensor Systems Based on Polystyrene Bead and Nanofibrous Solid Supports

Kunduru V., Prasad S., Patra P.K., Sengupta S., Portland State University, US

Polystyrene bead and electrospun polystyrene nanofibers are employed as subjects of study in this protein biomarker sensor. The study is conducted to compare the sensitivity and efficiency of the polystyrene nanofibers and microspheres as protein [...]

One-step Target Protein detection from Whole Blood in a Lab-on-a-Disc

Lee B-S, Lee J-G, Lee J.N., Lee B-S, Lee J-G, Lee J.N., Lee B-S, Lee J-G, Lee J.N., Park J.M., Cho Y-K, Kim S., Ko C., Samsung Advanced Institute of Technology, KR

We report a fully integrated, one-step Lab-on-a-Disc for detecting target protein from whole blood utilizing centrifugal microfluidics, so called “Immunoassay Lab-on-a-Disc”. The design principle is the microbead-based sandwich ELISA on the Disc. The microbeads are [...]