gene delivery – TechConnect Briefs

Functional Polylactide-based Systems for Delivery of Cancer Therapeutics

Cheng C., Sun H., Yu Y., Chen C-K, Cheng C., Zou J., University at Buffalo, the State University of New York, US

As an important class of biodegradable polymers, polylactides (PLAs) have been approved by FDA for clinical applications. However, conventional PLAs are lack of functionalities, and this significantly limits their applications. Therefore, in recent years we [...]

Self-assembling peptide dimers with multifunctionalities for gene delivery systems

Ryu K., Lee G.J., Kim T.I., Seoul National University, KR

Peptides have been used in various biomedical fields including gene and drug delivery systems [1]. There are many peptide sequences which can interact with cellular molecules or components. These biological interactions of peptide represented many [...]

Synthesis and evaluation of T85-LPEI copolymers for pDNA delivery

Wang M.X., Carolinas Medical Center, US

In this study, a series of low molecular weight PEI (LPEI) modified with Tween 85 were prepared by varying feed ratio and PEI size (Mw: 0.8k, 1.2k and 2.0k Da) for gene delivery. The Mws [...]

Cationic lipid nanoemulsions for RNAi screening

Bruniaux J., Sulpice E., Mittler F., Texier I., Gidrol X., Navarro F.P., CEA/LETI/MINATEC, FR

Since the discovery of RNA interference, many efforts have been made to broaden perspectives of small interfering RNA (siRNA) in research and clinical work. siRNA shows a specific and effective gene silencing activity through a [...]

Exploiting the “protein corona effect” for targeted drug and gene delivery

Caracciolo G., Pozzi D., Capriotti A.L., Cavaliere C., Cardarelli F., Salomone F., Laganà A., Sapienza University of Rome, IT

When administered in vivo lipid nanoparticles (NPs) are immediately covered by a rich protein layer, known as “protein corona” that changes the size, aggregation state, and interfacial properties of the NP, giving it a biological [...]

Self Assembled Nanostructures of Triblock Co-polymer and Plasmid DNA for Gene Delivery

Daima H.K., Shankar P.R. Selvakannan S., Bhargava S.K., Bansal V., RMIT University, AU

In present work, we have used tri-block copolymer (PEO-PPO-PEO) as a non-viral vector to deliver plasmid DNA (pDNA). Green fluorescence protein (GFP) plasmid DNA was used as a model plasmid DNA. Addition of pDNA to [...]

Surface Functionalized Multicomponent Envelope Type Nanosystems for Improved Gene Delivery

Pozzi D., Orlando V., Augusti Tocco G., Biagioni S., Cardarelli F., Bifone A., Caraccioloa G., Sapienza University of Rome, IT

Recently, a new programmed packaging strategy in which various “soft devices” that control intracellular trafficking are incorporated into single nanoparticles has been proposed. Here we apply such a novel concept to develop multicomponent envelope-type nanosystems [...]

Dual Modal Gene Expression and Silencing Using Viral/Nonviral Chimeric Nanoparticles for Synergistic Gene Therapy

Cho S.K., Luu T., Wong S., Kwon Y.J., UC Irvine, US

Efficient, safe, and versatile gene delivery carriers that combine the viral vectors’ high gene delivery efficiency and the nonviral vectors’ high level of flexibility for structural and functional modifications were prepared. Adeno-associated virus (AAV) was [...]

Layer-by-Layer Deposition of Cationic Polymers on Gold Nanoparticle for Non-viral Gene Delivery System

Saengkrit N., National Nanotechnology Center (NANOTEC), TH

In this study, gold nanoparticles displayed spherical shapes with an average size of 12 nm were synthesized. The nano-carriers for gene delivery were subsequently developed based on DNA adsorption on the gold/cationic polymer-scaffold. The multilayer [...]

Molecular dynamic simulations of bio-nanocomplexes involfing LDH nanoparticles and short-strand DNA/RNA

Tran P., Wong Y., Lu G.Q., Smith S.C., University of Queensland, AU

A variety of organic structures can be intercalated into layered double hydroxides (LDH) structures, making LDH nanoparticles highly promising vehicles for drug delivery and gene therapy applications. The nature of the interactions between DNA (or [...]