gene delivery – Page 2 – TechConnect Briefs

Modeling Magnetic Transport of Nanoparticles with Application to Magnetofection

Furlani E.P., Ng K.C., University at Buffalo, US

Magnetic nanoparticles are finding increasing use in a broad range of bioapplications, primarily as carrier particles for biomaterials such as cells, proteins, antigens and DNA. The use of biofunctional magnetic particles enables selective immobilization and [...]

Non-Condensing Calcium Alginate Microspheres for Macrophage-Selective Gene Delivery and Transfection

Jain S., Amiji M., Northeastern University, US

The main objective of the study was to design a non-condensing polymer microsphere-based gene delivery system for macrophages. An external gelation technique was used to prepare plasmid DNA-encapsulated calcium alginate microspheres. The microspheres were characterized [...]

Organisation of peptide ligands on the surface of gene vectors

Wang M., Miller A.D., Thanou M., Imperial College London, UK

Through the considerate organisation of surface ligands, we have developed a lipid-based vector for the targeted delivery to cancer cells. Our ligand is a short peptide with high affinity for the Urokinase Plasminogen Activator receptor [...]

Gene Delivery and Transfection Studies with Lipopolyplexes in Human Endothelial and Smooth Muscle Cells

Brito L., Little S., Langer R., Amiji M., Northeastern University, US

Non-viral gene delivery and transfection in primary human vascular endothelial cells (EC) and smooth muscle cells (SMC) has tremendous potential for cardiovascular diseases, such as in treatment of coronary restenosis. Using a combination of synthetic [...]

Polymer-based gene delivery system fabricated with the participation of a polyanion

Sun Q., Stolnik S., University of Nottingham, UK

A DNA/polycation/polyanion system is being designed, which is supposed to be a potentially improved system in comparison to the traditional gene delivery system (DNA/polycation system). The production of such a DNA/polycation/polyanion system is based on [...]

Condensation of Therapeutic Oligodeoxynucleotides and Plasmid DNA with PPI Dendrimers and PPI-Modified Gold Nanoparticles

Chen A.M., Nair S.K., Thomas T.J., Thomas T.T., Thomas T.J., Thomas T.T., He H., Rutgers Unverisity, Newark, US

We demonstrated the formation of oligonucleotide nanoparticle in the presence of polypropylenimine dendrimers using total intensity light scattering as well as AFM and TEM. Confocal microscopic analysis showed that the nanoparticles formed with G4 and [...]

Understanding the Cytoplasmic Transport of Synthetic Gene Vectors

Pangarkar C., Dinh A.T., Mitragotri S., university of california, santa barbara, US

We use fluorescence video microscopy and single particle tracking to elucidate some of the fundamental mechanisms involved in intracellular trafficking of non-viral gene vectors. Transport of polymer-DNA complex, which are internalized via endocytosis, is almost [...]

Ca-Mg Phosphate Nano-Crystals for High Efficiency Gene Delivery and Expression in Mammalian Cells

Chowdhury E.H., Akaike T., Tokyo Institute of Technology, JP

Significant efforts are now being made for the development of non-viral gene-delivery techniques as alternatives to the viral vectors for basic research and clinical medicine. Despite existence of a wide variety of non-viral techniques particularly [...]

Enhanced Gene Delivery Efficiency in Resonant Acoustic Fields

Lee Y-H, Peng C-A, University of Southern California, US

Enhancement of DNA delivery efficiency has been improved by several physical and chemical approaches in the past decade. However, the application of those methods is hampered by large-scaled configurations. To augment gene delivery efficiency with [...]

Structural Characterization of Novel Micro- And Nano-Scale Non-Viral DNA Delivery Systems for Cutaneous Gene Therapy

Foldvari M., Badea I., Wettig S., Verrall R., Bagonluri M., University of Saskatchewan, CA

Our main goal is to develop micro/nano-scale self-assembling delivery vehicles for cutaneous gene therapy. The objective of this study was to characterize the structural and physicochemical properties of novel dicationic lipid-based DNA complexes by atomic [...]