protein – Page 2 – TechConnect Briefs

Counting and Sizing of Virus and Protein Aggregates by Nanoparticle Tracking Analysis (NTA)

Griffiths D., Hole P., Smith J., Malloy A., Carr B., NanoSight, US

A new analytical system is described which provides total particle count and high-resolution size measurements for biological structures such as virus and protein aggregates. This system builds on the Nanoparticle Tracking and Analysis (NTA) technique [...]

‘Lab on a chip’ Label Free Protein Sensor Systems Based on Polystyrene Bead and Nanofibrous Solid Supports

Kunduru V., Prasad S., Patra P.K., Sengupta S., Portland State University, US

Polystyrene bead and electrospun polystyrene nanofibers are employed as subjects of study in this protein biomarker sensor. The study is conducted to compare the sensitivity and efficiency of the polystyrene nanofibers and microspheres as protein [...]

Biomolecular activity switch: an application of metallic nanoparticle plasmon resonance

Alper J., Wijaya A., Crespo M., DeFlores L., Tokmakoff A., Hamad-Schifferli K., Massachusetts Institute of Technology, US

Specific external control of biomolecular activity is a critical step in harnessing the power of nature’s molecular machinery. We propose and demonstrate one control method, a biomolecular activity switch. Since proteins are the primary macromolecule [...]

Nanostructured Biomolecular Assemblies Based on DNA-Protein Interactions

Sroga G.E., Dordick J.S., Rensselaer Polytechnic Institute, US

Directed, biologically-driven self-assembly has the potential to yield hybrid multicomponent architectures with applications ranging from nanoelectronic devices to smart drug delivery systems and responsive materials. To enable these applications, it is critical to gain control [...]

Manufacturable nanoscale membranes – material development and biological separations

Gaborski T.R., Striemer C.C., Snyder J.L., Fang D.Z., Fauchet P.M., McGrath J.L., University of Rochester, US

We have developed a new class of ultrathin porous nanocrystalline silicon (pnc-Si) membranes using inexpensive and scalable silicon fabrication techniques. Pnc-Si is formed from a deposited amorphous silicon thin film, where nanoscale pores are introduced [...]

The Si-tag for Immobilizing Proteins on a Silicon Device

Kuroda A., Hiroshima University, JP

Targeting functional proteins to specific sites on a silicon device is essential for the development of new biosensors and supramolecular assemblies. Recently, we found ribosomal protein L2 as a silicon-binding protein, which can be used [...]

Label-Free Protein Nano-Biosensor Using Top-Down Fabrication

Ah C.S., Ahn C-G, Kim A., Yu H.Y., Ahn C-G, Yang J.H., Baek I-B, Park C.W., Jun M.S., Lee S.J., Electronics and Telecommunications Research Institute (ETRI), KR

A nanosensor of n-type Si field-effect transistor (FET) via “top-down method” was employed to monitor PSA with great selectivity and high sensitivity up to 10 pg/mL. When a biomaterial, negatively charged PSA, is introduced through [...]

Nanoelectrodes with Selective Molecular Receptors for Label-Free Electronic Detection of Thrombin

Dong J., Parviz B.A., University of Washington, US

The enzyme thrombin plays a crucial role in the regulation of blood coagulation. We present a sensor that can directly detect this enzyme and generate an electronic signal. The sensor structure consists of three electrodes [...]

Denaturation of hen egg white lysozyme in electromagnetic fields: a molecular dynamics study

English N.J., Mooney D.A., University College Dublin, IE

Nonequilibrium molecular dynamics simulations of hen egg white lysozyme have been performed in the canonical ensemble at 298 K in the presence of external electromagnetic fields of varying intensity in the microwave to far-infrared frequency [...]

Protein can act as unique template to assemble gold nanoparticles in a linear fashion

Bhattacharya R., Patra C.R., Wang S., Lu L., Yaszemski M.J., Mukhopadhyay D., Mukherjee P., Mayo Clinic, US

Nanoparticles of metals possess unique chemical, electronic, optical and magnetic properties from their bulk counterpart because of the quantum size effects. The spatial orientation and arrangement of nanoparticles is important in the realization of technologically [...]