protein – TechConnect Briefs

Bioextraction technologies for bioplastic and biomass hydrolysates production

Hall E., Ibrahim M.H.A., Bioextrax AB, SE

Globally, there is an increased interest in environmentally friendly bio-based alternatives to currently used chemical processes in agriculture, medicine and industry. Microorganism and its enzymes can provide safe and cost-effective technologies for different applications. One [...]

Effect of silver coated carbon nanotubes on metabolically essential gene expression and outer membrane protein profile of Escherichia coli

Chaudhari A.A., Pillai S., Singh S.R., Alabama State University, US

Antibiotic resistance acquired by food borne pathogens such as E. coli has become a global concern. Recently, nanoparticles have been shown to be effective alternatives to antibiotics. Specifically, silver coated carbon nanotubes (AgCNTs) have been [...]

Neutron Scattering for Biological Research: Progress at the Bio-SANS Beam Line

Langan P., O'Neill H.M., Pingali S.V., Qian S., Urban V., Weiss K., Oak Ridge National Laboratory, US

Structure-function relationships remain a critical theme in understanding many important biological processes regarding energy, disease and other applications. At Oak Ridge National Laboratory, home to two of the most powerful neutron sources for research High [...]

Hydroxylamine-O-sulfonic acid as a New Reducing Agent for the Formation of Nearly Monodisperse Gold Nanoparticles in water: Synthesis Characterisation and Bioconjugation

Holmes J.D., Rahme K., Notre Dame University (Louaize), LB

Gold nanoparticles (Au NPs), with diameters ranging between 60-150 nm, have been synthesised in water at room temperature by reducing HAuCl4.3H2O with hydroxylamine-o-sulfonic acid in the presence of sodium citrate, as stabilising agent. 15 and [...]

Challenges in the Characterisation of Therapeutic Protein Formulations

Ball S., Clarke P., Murphy C., Pothecary M., Malvern Instruments, UK

In recent years, the stability of proteins in solution has attracted significant scientific and commercial interest due to the exponential growth in the use of proteins as biotherapeutic drugs. Since knowledge of formulation stability and [...]

Encapsulation and Characterization of BSA in PLA-PEG Nanoparticles as a Model for Chlamydia trachomatis rMOMP-278 Protein Nanovaccine Delivery System

Agee R., Dennis V.A., Moore C., Singh S.R., Taha M., Alabama State University, US

Chlamydia trachomatis is a major human health pathogen because it is the most reported sexually transmitted disease. There is no effective mucosal vaccine against Chlamydia. One of the major problems of the C.trachomatis vaccine development [...]

Atomistic Simulations of Biomolecules at the Water‐Amorphous Silica Interface: Application to Peptides and DNA Oligomers

Hassanali A., Shi B., Shin Y.K., Singer S.J., Ohio State University, US

Realistic modeling of biomolecules near amorphous silica (“glass”), a material commonly used for biomedical device fabrication, is required for device design and evaluation. Our potential model, based on ab initio quantum chemical calculations, is designed [...]

Nano-plamsonics Sensing and Integration with Microfluidics for a Lab-on-chip Biosensor

Kofke M., Rexius M., Singhal S., Waldeck D.H., Wang Y., Wei J.J., CFD Research Corporation, US

Ordered arrays of nanostructures in metal films have been studied for practical miniaturization of SPR sensing and microfluidic integration. We report a nanostructure array that is designed to permit significantly enhanced Extraordinary Optical Transmission (EOT) [...]

Mutations of cypovirus polyhedrin and applications of polyhedra to protein nanocontainers

Chiu E., Coulibaly F., Metcalf P., Mori H., Nakai D., Ohtsuka Y., Kyoto Institute of Technology, JP

Cypoviruses, a member of the family Reoviridae, are one group of insect virus that produce micrometer-sized protein crystals called cytoplasmic polyhedra. Many virus particles are occluded in polyhedra to protect them against extracellular environment. Recently [...]

Application of insect virus polyhedra to protein nanocontainers

Ijiri H., Kotani E., Mori H., Kyoto Institute of Technology, JP

Certain insect viruses encode a protein called polyhedrin that forms polyhedric crystals, or polyhedra, within an infected cell. The virus particles, or virions, are protected within these polyhedra and can remain infectious for years outside [...]