protein – Page 3 – TechConnect Briefs

Mounting proteins on metal nanoparticles: Statistical analysis of AFM images

Gurevich L., Fojan P., Saxena R.K., Petersen S.B., Aalborg University, DK

In this work we assess the use of atomic force microscopy (AFM) study combined with activity assays, DLS and fluorescent measurements for complex characterization of nanoparticle-protein conjugates. Image processing techniques were used to locate and [...]

Preservation of DNA and Protein Biofunctionality for Bio-MEMS & NEMS Fabrication

Trau D., Jie J., Sucher N.J., National University of Singapore (NUS), SG

Microfabrication processes especially in silicon are not compatible with biomolecules. For example, silicon processing is commonly performed at high temperatures and makes use of aggressive chemicals. Under these conditions biological materials are denaturated or decomposed. [...]

Enhancement of E6 Protein Binding on Binding-Orientation-Sensitive Mixed SAMs Molecules

Tseng F-G, Tseng Y.T., Hwang J.K., Hung S.W., Chang J.M., Huang H.M., Huang T.W., Tseng F-G, Tseng Y.T., Huang H.M., Huang T.W., Chieng C-C, National Tsing Hua University, TW

Orientation selectivity and binding enhancement with E6 protein are demonstrated by nano-structured mixed-SAMs systems of different chain lengths. Mixtures of n-alkinethiol SAMs of different chain lengths: 1-Decanethiol (C9) and 1-Hexanethiol (C5) are the examples in [...]

New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules

Snabe T., Neves-Petersen M.T., Fojan P., Klitgaard S., Neves-Petersen M.T., Petersen S.B., Aalborg University, DK

Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized [...]

Boolean Networks (BN) Based Molecular Biology Modeling: Protein Process

Yeol J.W., Barjis I., Barjis J., Barjis I., Barjis J., Berri S., Ryu Y., Polytechnic University, US

In this paper, we introduce a new application of modeling tool; Boolean Networks (BN). In particular, it shows how, by means of a Boolean Networks (BN), the protein production process can be modeled and analyzed. [...]

Imaging Ellipsometry: A Powerful Method for Biochip Development, QC and Evaluation

Laging M., Hoenig D., Eing A., Accurion, US

Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent [...]

Self-Assembly of Gold Nanoparticles on E-Beam Nano-Patterns Towards Protein Nanoarray

Powell T., Yoon J-Y, University of Arizona, US

Self-assembly of gold nanoparticles on e-beam nano-patterns towards protein nanoarray. A gold colloid suspensions containing different size nanoparticles, will be dumped in succesion, and the particles will self assemble into proper sized trenches.

Biomolecular Patterning via Photocatalytic Lithography

Bearinger J.P., Hiddessen A.L., Wu K.J.J., Christian A.T., Dugan L.C., Stone G., Camarero J., Hinz A.K., Hubbell J.A., Lawrence Livermore National Laboratory, US

We have developed a novel method for patterning surface chemistry: Photocatalytic Lithography. This technique relies on inexpensive stamp materials and light; it does not necessitate mass transport or specified substrates, and the wavelength of light [...]

Protein Nanoparticles

Castor T.P., Aphios Corporation, US

Protein nanoparticles can be utilized for the pulmonary delivery of protein therapeutics or can be incorporated into biodegradable polymer microspheres/nanospheres for controlled release depot or oral delivery. With proteinaceous therapeutics, the generation of nanoparticles is [...]

Development of an Advanced Simulation System for the Analysis of Particle Dynamics in LASER based Protein Ion Sources

Hieke A., Ciphergen Biosystems, Inc., US

An advanced multi-physics simulation system henceforth referred to as GEMIOS (Gas and Electromagnetic Ion Optical Simulator) has been implemented which allows the computation of 3D trajectories and energy exchange of ion beams/clouds under the influence [...]