Papers – Page 38 – TechConnect Briefs

Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip

Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]

Sample-Shunting Based PCR Microfluidic Device

Auroux P-A, Day P.J.R., Manz A., Imperial College, UK

A new method to perform on-chip DNA amplification is presented in this abstract: we shunt the sample back and forth over temperature-controlled zones. Compared to existing systems, this approach offers, in addition to a greater [...]

Visualizing Individual Rhodopsin (a G Protein-Coupled Receptor) Molecules in Native Disk and Reconstituted Membranes via Atomic Force Microscopy

Choi E.J., Jin A.J., Niu S-L, Smith P.D., Litman B.J., National Institutes of Health, US

Individual rhodopsin molecules have been resolved with atomic force microscopy as both monomers and various oligomeric organizations that are sensitively dependent upon the physical state of membranes and environmental conditions. In intact native disk membranes, [...]

Composable System Simulation of Dispersion in Complex Electrophoretic Separation Microchips

Wang Y., Lin Q., Mukherjee T., Carnegie Mellon University, US

This paper presents a composable system simulation framework for electrophoretic separation microchips, using an analog hardware description language integrating analytical dispersion models that describe not only the behavior of individual components, but also the interactions [...]

Development of a Slug-Flow PCR Chip with Minimum Heating Cycle Times

Hardt S., Dadic D., Doffing F., Drese K.S., Münchow G., Sörensen O., Institute of Microtechnology Mainz, DE

A microfluidic PCR device is presented capable of rapid temperature ramping and handling of sample volumes in the microliter and submicroliter range. The PCR chip comprises a straight micro channel in which a sample slug [...]

Microfabricated Fluorescence-Activated Cell Sorter with Hydrodynamic Flow Manipulation

Bang H., Chung C., Chung S., Kim J.K., Kim S., Kim J.K., Kim S., Chung C., Chung S., Han D-C, Chang J.K., School of Mechanical & Aerospace Eng., Seoul National University, KR

Presented is a novel flow manipulation and particle detection method for the microfabricated fluorescence-activated cell sorter (FACS). With hydrodynamic flow manipulation which includes passive flow channeling and hydrodynamic actuation with nozzle flow, we have developed [...]

High-Resolution Electrochemical In Situ Printing

Egeland R.D., Southern E.M., Oxamer & Oxford Gene Technology, UK

This work demonstrates a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass [...]

An Chip-based-Instant Protein Micro Array Formation and Detection System

Tseng F-G, Ho C.E., Chen M.H., Chen Y.F., Hung K.Y., Su C-J, Chen M.H., Chen Y.F., Huang H.M., Chieng C-C, National Tsing Hua University, TW

This paper proposes a novel protein micro arrayer employing 3-in-1 chip system, instantly delivering hundreds of biosamples in parallel for disease diagnosis. The 3-in-1chip system included: micro filling chip, micro stamper array and micro bio-reaction [...]

Functional Nanoarrays for Protein Biomarker Profiling

Lynch M., Mosher C., Huff J., Nettikadan S., Xu J., Henderson E., BioForce Nanosciences, Inc., US

The success of microarrays for analyzing nucleic acid and, more recently, protein-protein interactions illustrates the advantages of bioassay miniaturization. However, the current level of miniaturization of useful bioanalytical tools still creates limits in terms of [...]

Detection of DNA Hybridyzation with Impedance Amplifying Labels

Ma K-S, Madou M., Zhou H., Fischetti A., Zoval J., Madou M., University of California Irvine, US

Traditionally used nucleic acid analysis detection platforms rely on fluorescent or radioactive labels. Optical techniques require large and expensive equipment such as automated colorimetric and fluorimetric instruments that greatly increase the cost of the whole [...]