Papers – Page 37 – TechConnect Briefs

A Molecular Motor that Links the Biological and Silicon Worlds

Robinson T., Blundell A., Dutta C.F., Firman K., University of Portsmouth, UK

The EcoR124I restriction endonuclease is also a biological molecular motor. It is able to attach to DNA at a specific location and then translocate the rest of the DNA molecule through the bound complex. Therefore, [...]

Actin Adsorbed on HOPG and Mica Substrates: Characterization and Manipulation by Atomic Force Microscopy

Nicolau D.V., Watson G.S., Cahill C., Blach J., Myhra S., Swinburne University, AU

We report on the fabrication of nanostructures built of actin using the Atomic Force Microscopy and molecularly organised substrata, i.e. HOPG and mica. We obtained both ‘paracrystalline rafts’ of actin adsorbed onto hydrophobic HOPG, as [...]

Evaluation of Electric Impedance Spectra for Single Bio-Cells with Microfluidic Devices using Combined FEMLAB/HSPICE Simulated Models

Senez V., Yamamoto T., Poussard B., Fukuba T., Capron J.M., Fujii T., LIMMS/CNRS-IIS - University of Tokyo, JP

This paper describes a simple method to predict the electrical impedance spectrum of single & cultured cells in micro-devices. It can be used for the rapid design of micro-sensors as well as for more fundamental [...]

Amplification of Protein Adsorption on Micro/Nanostructures for Microarray Applications

Ivanova E.P., Alekseeva Y.V., Pham D.K., Filipponi L., Nicolau D.V., Swinburne University, AU

The fabrication and operation of biodevices require the accurate control of the concentration of the bioactive molecules on the surface, as well as the preservation of their bioactivity, in particular proteins that have a significant [...]

Development and Characterization of a 2-D microchip-based Protein separation system

Feng J.J., Olazábal V, Olivares J.A., Sundaram S., Krishnamoorthy S., Iyer S., Los Alamos National Laboratory, US

Traditional methods in proteomics have relied on gel-based separation of proteins followed by directed cleavage of these proteins and mass spectrometry to measure the products. The emphasis on reliable, high-throughput analyses has led to the [...]

Molecular Simulation of DNA Microarrays – an Application of 2D particle mesh Ewald algorithm

Watanabe T., Kawata M., Nagashima U., National Institute of Advanced Industrial and Science and Technology, JP

We have developed the two-dimensional particle mesh Ewald (2D-PME) algorithm for molecular dynamics (MD) simulation, and applied it to have knowledge of the behavior of DNA and its interaction on microarrays, which crucial in the [...]

Dry Microarrays: A Generalized Data Source for Genome and Proteome Analysis

Sampath G., The College of New Jersey, US

Microarray analysis methods can be applied to characteristic data obtained from biological sequences in matrix form (which could include data from experiments or bioassays as well). The rows correspond to a genome region (intron or [...]

Dielectrophoresis (DEP) of Cells and Microparticle in PDMS Microfluidic Channels

Kanagasabapathi T.T., Backhouse C.J., Kaler K.V.I.S., University of Calgary, CA

Dielectrophoresis (DEP) [1] The behavior of polarized, neutral particles to the non-uniform electric field has been successfully applied for the levitation and subsequent isolation of biological cells and other micron-sized inorganic particles [2, 3]. This [...]

An Injector Component Model for Complete Microfluidic Electrokinetic Separation Systems

Magargle R., Hoburg J.F., Mukherjee T., Carnegie Mellon University, US

This work presents a closed-form, numerically derived model of a key microfluidic electrokinetic separation system component, the cross injector. The model fits into a framework such that it can be combined with a network of [...]

Effect of Substratum Morphology on Cell Behavior

Su W-T, Chu I-M, Yang J-Y, Lin C-D, National Nano Device Laboratories, TW

Contact guidance induced by the surface topography of the substrata influences the direction of cells with artificial material. Mouse bone marrow stromal fibroblast was examined on grooved substrata of varying dimension (0.3£gm~1.2£gm groove width, 0.5£gm [...]