Sample-Shunting Based PCR Microfluidic Device

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A new method to perform on-chip DNA amplification is presented in this abstract: we shunt the sample back and forth over temperature-controlled zones. Compared to existing systems, this approach offers, in addition to a greater flexibility in terms of cycling programme, an incomparable ease of parallelisation. We report in this paper the first results obtained when using a sample-shunting approach to achieve on-chip PCR. An unoptimised chip displays an efficiency of 25% and is six times faster than a conventional thermo-cycler. To the extent of our knowledge no comparable results have been previously published.

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Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: March 7, 2004
Pages: 67 - 69
Industry sector: Medical & Biotech
Topicss: Biomaterials, Materials Characterization & Imaging
ISBN: 0-9728422-7-6