TechConnect Proceedings Papers
Month: May 2005
Self-Assembly of Gold Nanoparticles on E-Beam Nano-Patterns Towards Protein Nanoarray
Self-assembly of gold nanoparticles on e-beam nano-patterns towards protein nanoarray. A gold colloid suspensions containing different size nanoparticles, will be dumped in succesion, and the particles will self assemble into proper sized trenches.
Ultramicro Protein Arrays for Cytokine Detection
Radke K., Ryckman K., Nettikadan S., Lynch M., Mosher C., Xu J., Henderson E., BioForce Nanosciences, Inc., US
Several solid phase microarray based methods for the detection of the genomic materials have been developed based on amplifying the target molecules. Since proteins cannot be amplified, a similar approach to protein detection faces some [...]
Enhancement of DNA Microarray Hybridization using Shear-Driven Microfluidics
Vanderhoeven J., Pappaert K., Dutta B., Vanhummelen P., Baron G.V., Desmet G., Free University of Brussels, BE
On microarrays the transport of the target molecules towards the spots only occurs by molecular diffusion. As a result, microarray analyses require overnight incubation and are highly inefficient in terms of capture rate and detection [...]
Biomolecular Patterning via Photocatalytic Lithography
Bearinger J.P., Hiddessen A.L., Wu K.J.J., Christian A.T., Dugan L.C., Stone G., Camarero J., Hinz A.K., Hubbell J.A., Lawrence Livermore National Laboratory, US
We have developed a novel method for patterning surface chemistry: Photocatalytic Lithography. This technique relies on inexpensive stamp materials and light; it does not necessitate mass transport or specified substrates, and the wavelength of light [...]
The Large Scale Fabrication of Protein Nanoarrays Based on Nanosphere Lithography
Many emerging biological and medical applications involve the development of protein arrays at nanoscale.1 Dip-pen lithography2 nanografting and conducting tip AFM writing are capable of creating nano-protein patterns with 50-100 nm resolution over small surface [...]
Fast DNA Hybridization on a Multi-Sample Multi-probe Microfluidic Microarray Compact Disc
Peng X.Y., Li P.C.H., Yu H-Z, Parameswaran A.M., Beardmore S., Chou W.L., Simon Fraser University, CA
DNA microarrays are usually constructed using photolithographic synthesis of oligonucleotide probes or by spotting of pre-synthesized probes. These are expensive methods, and only one sample can be applied on a microarray chip at one time. [...]
Superparamagnetic Iron Oxide-Gold Core-Shell Nanoparticles for Biomedical Applications
Iron oxide nanoparticles (NPs) in the form of maghemite and magnetite have been used for targeted drug delivery, magnetic cell sorting, magnetic resonance imaging (MRI) contrast agents and immunoassays. Surface modification of these NPs is [...]
Hetero-bifunctional Poly(ethylene glycol) Modified Gold Nanoparticles as an Intracellular Tracking and Delivery Agent
Fu W., Shenoy D., Li J., Crasto C., Jones G., Dimarzio C., Sridhar S., Amiji M., Northeastern University, US
To increase the targeting potential, circulation time, and the flexibility of surface-attached biomedically-relevant ligands on gold nanoparticles, hetero-bifunctional poly(ethylene glycol) (PEG, MW 1,500) was synthesized having a thiol group on one terminus and a reactive [...]
Phosphorylcholine-protected Nanoparticles Functionalized with Both Magnetic and Photoluminescent Properties
We prepared the magnetite core coated with nano-graded ZnS shell to display superparamagnetic and photoluminescent properties. By adsorbing the phosphorylcholine-ended thiol to stabilize the core-shell nanoparticles, the particles can be well-dispersed in aqueous buffer solution [...]