PCR – TechConnect Briefs

Bacillus Biocathode improves Electricity Generation with Microbial Fuel Cells

Han S., Zhou C., Wang R., Missouri University of Science and Technology, US

In a microbial fuel cell (MFC), biocathodes alleviate the need to use noble catalysts to reduce oxygen, which substantially increases the viability and sustainability of a MFC. Three electrochemically active strains of bacteria are isolated [...]

Magnetic Nano Particles for Diagnostics

Nguyen K.C., Pham V.H., Do S.T., Tieu D.T., SAIGON HI TECH PARK, VN

Magnetic Nano Particles (MNP) for diagnostics had been fabricated by coating the particle ‘s surface with specific coupling agents . This agent allows the pulling the virus containing DNA and/or RNA molecules off the patient [...]

DNA Amplification by PCR using Low cost, Programmable Microwave Heating

Garg P., Hella M., Borca-Tasiuc D.-A., Mohamed H., Rensselaer Poytechnic Institute, US

This work presents a low power microstrip transmission line PCR thermo-cycler based on microwave heating that is designed for low-budget, portable/Point of Care (POC) genetic analysis. Previous efforts on miniaturized PCR chambers focused mainly on [...]

Novel piezo motor enables positive displacement microfluidic pump

Henderson D., New Scale Technologies, US

Microfluidic pumping mechanisms, such as membrane micro pumps, create a pulsed stream with generally low pressure, low flow and limited precision. We present a novel piezoelectric motor that is used to create a positive displacement [...]

Fast and Efficient Nucleic Acid Testing by ST‘s In-CheckTM Lab-on-Chip Platform

Conoci S., Di Francesco F., Di Pietro P., Di Trapani G., Petralia S., Amore M.G., San Biagio F., Alaimo G., Iacono G., Alessi E., Ricceri D., Di Francesco F., Di Pietro P., Di Trapani G., Di Francesco F., Di Pietro P., Di Trapani G., Palmieri M., STMicroelectronics, IT

Nucleic Acid Testing (NAT) represents one the most sensitive and effective method for several applications in the molecular diagnostic area, including clinical, industrial, veterinary and research applications. In this context, the development of miniaturized devices [...]

Rapid DNA Amplification in Buoyancy Driven Closed Loop Microfluidic Systems

Agrawal N., Ugaz V.M., Texas A&M University, US

Buoyancy driven fluid motion, however, can also occur in closed loop geometries in response to either vertically or horizontally imposed temperature gradients. These systems offer the exciting possibility of generating unidirectional laminar flows along a [...]

Flat Fluidics: Programmable On-Chip Networks for Biological and Chemical Applications

Wixforth A., University of Augsburg and Advalytix AG, DE

We present a novel approach towards the needs of a versatile microfluidic chip-based microfluidic system with unique properties and functionality. Like for microarrays and in contrast to many existing microfluidic technologies, the fluid handling is [...]

Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA

Li H.X., Rothberg L.J., University of Rochester, US

Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all [...]

Lattice Boltzmann Simulations Comparing Conventional and a Heat Conduction Based Flow-Through PCR Micro-Devices

Mautner T., SPAWAR Systems Center San Diego, US

This paper describes the lattice Boltzmann, numerical simu-lations used to evaluate a conduction-based, polymerase chain reaction (PCR) device for uniformity of the micro channel temperature profile and its velocity field distribu-tion. For comparison purposes, numerical [...]

Sample-Shunting Based PCR Microfluidic Device

Auroux P-A, Day P.J.R., Manz A., Imperial College, UK

A new method to perform on-chip DNA amplification is presented in this abstract: we shunt the sample back and forth over temperature-controlled zones. Compared to existing systems, this approach offers, in addition to a greater [...]