PCR – Page 2 – TechConnect Briefs

Development of a Slug-Flow PCR Chip with Minimum Heating Cycle Times

Hardt S., Dadic D., Doffing F., Drese K.S., Münchow G., Sörensen O., Institute of Microtechnology Mainz, DE

A microfluidic PCR device is presented capable of rapid temperature ramping and handling of sample volumes in the microliter and submicroliter range. The PCR chip comprises a straight micro channel in which a sample slug [...]

Highly Integrated Transparent Microchannel Chips for Continuous-flow PCR

Sun K., Matsuo S., Misawa H., Satellite Venture Business Laboratory, The University of Tokushima, JP

A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques, and was sealed by another quartz [...]

PCR Micro-Voume Device for Detection of Nucleic Acids

Auroux P-A, Day P., Niggli F., Manz A., Kinderspital, CH

The polymerase chain reaction (PCR) is an enzyme-mediated nucleic acid amplifer process that is employed extensively throughout the medical and biological sciences. The technique has risen to acclaim largely through its exquisite sensitivity, but an [...]

System Performance Evaluation with SystemC for Two PCR Microelectrofluidic Systems

Zhang T., Chakrabarty K., Fair R.B., Cadence Design Systems, Inc., US

We present a performance comparison between two types of microfluidic systems - continuous-flow systems and droplet-based systems. The comparison based on a specific microfluidic application - a polymerase chain reaction (PCR) system. The modeling and [...]

Simulation and Experimental Verification of Micro Polymerase Chain Reaction Chip

Lin Y-C., Yang C-C., Hwang M-Y., Chang Y-T., Cheng Kung University, TW

This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, [...]