Diagnostics & Bioimaging – Page 24

Diode Laser Generated Ultrasound for Human Blood Cell Lysis

Ussing T., von Gersdorff L.C., FluimediX, DK

The lysing of human blood leukocyte cells prior to performing e.g. DNA analysis is performed on a routine basis in most biochemical labs. Well-established techniques include using a lysis agent or using mechanical methods such [...]

Lab-on-chip for the isolation and characterization of circulating tumor cells

Laureyn W., Liu C., O’Sullivan C.K., Llaudet E., Nilsson O., Drese K.S., Roeser T., Ritzi M., Hauch S., Albert W., Borgen E., Nygren A.O.H., IMEC, BE

A consortium of 7 European partners has undertaken the challenging task to develop a microfluidic system (μTAS) capable of the isolation/enrichment and consecutive RNA/DNA characterization of circulating tumor cells (CTCs), to be used as a [...]

Noise-Differential Based Detection of Cells on a Microelectronic Sensor/Actuator Array

Manaresi N., Lanzetta F., Romani A., Medoro G., Guerrieri R., Silicon Biosystems spa, IT

We present a new method to count cells on a microelectonic lab-on-a-chip integrating an array of Dielectrophoretic (DEP) cages and embedded optical sensors. Cells are trapped by DEP cages above photodiodes measuring the optical near [...]

One-step Pathogen Specific DNA Extraction from Whole Blood On a Centrifugal Microfluidic Device

Cho Y-K, Lee B-S, Lee J-G, Lee Y., Park J.M., Lee B-S, Lee J-G, Lee Y., Lee B-S, Lee J-G, Lee Y., Ko C., Samsung Advanced Institute of Technology, KR

We report a fully integrated pathogen specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. Using the innovative Laser Irradiated Ferrowax Microvalves (LIFM) together with the rapid cell lysis method using [...]

Devices for Nanoparticle-based Cancer Detection

Kim G.Y., Daniel K.D., Vassiliou C.C., Elman N., Josephson L., Cima M.J., MIT, US

This implantable biomarker sensor combines nanoscale magnetic relaxation switches (MRSW) and the versatility of microfabricated structures tp be read by MRI. MRSW are magnetic iron oxide nanoparticles that can be functionalized to detect a variety [...]

Microfluidic electromanipulation with capacitive detection for cell diagnostic applications

Ferrier G.A., Hladio A.N., Thomson D.J., Bridges G.E., Hedayatipoor M., Olson S., Freeman M., University of Manitoba, CA

We are proposing an all-electrical approach to mechanical cell diagnostics that uses a combination of electromanipulation for stimulus and capacitance for sensing. We have successfully used the capacitive sensor to detect the electrical trapping (using [...]

Investigation of pH behavior of single myocyte cells in picoliter microfluidic device

Ges I.A., Baudenbacher F.J., Vanderbilt University, US

The possibility to investigate single cell metabolic activity in real time in sub-nanoliter volume could expand our knowledge of cellular physiological processes. Extracellular pH is a critical parameter for many cellular processes and extracellular acidification [...]

Nanobiopore: A Novel Nano-Porous Electrode System to Enhance Biosensor Sensitivity

Müller U., Nisch W., Neugebauer S., Schuhmann W., Linke S., Kaczor M., Lohmüller T., Spatz J., Motz M., Sorsa J., Hecke S., Hartwich G., Stelzle M., NMI Natural and Medical Sciences Institute, DE

The primary objective of this research is to create electrode systems for use as electrochemical transducers with enhanced sensitivity in order to enable detection of low concentration diagnostic targets not accessible to date. NanoBioPore sensors [...]

High-sensitive Label-free Biosensors Based on Carbon Nanotube Field-effect Transistors Modified with Aptamers

Maehashi K., Katsura T., Matsumoto K., Kerman K., Takamura Y., Tamiya E., Osaka University, JP

We have fabricated label-free protein biosensors based on aptamer-modified carbon nanotube field-effect transistors (CNTFET) for detection of immunoglobulin E (IgE). Since aptamers are artificial oligonucleotides, the aptamers are smaller in size than the Debye length. [...]

Selective Heating Characterization of Nanoplate Devices for Simultaneous Sensing of Multiple Biomolecule Species at Ultra-low Concentrations

Elibol O.H., Reddy_Jr. B., Bashir R., Purdue University, US

We propose a novel scheme for the en masse fabrication and selective functionalization of field effect silicon nanoplate devices to enable the ultrasensitive, simultaneous and label-free detection of multiple biomolecular species in real time. The [...]