One-step Pathogen Specific DNA Extraction from Whole Blood On a Centrifugal Microfluidic Device

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We report a fully integrated pathogen specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. Using the innovative Laser Irradiated Ferrowax Microvalves (LIFM) together with the rapid cell lysis method using laser irradiation on magnetic particles, we could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood on a CD. As a model study, DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) and E.coli were conducted. The total process of the plasma separation, mixing with magnetic beads conjugated with target specific antibodies, removal of plasma residual, washing, and DNA extraction was finished within 12 minutes with only one manual step of loading 100 microliters of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD was as good as that of the samples prepared in conventional bench top method. It demonstrates that our novel centrifugal microfluidics design enables a full integration of complex biological reactions that require multi-step fluidic control.

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Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 221 - 224
Industry sector: Medical & Biotech
Topic: Diagnostics & Bioimaging
ISBN: 1-4200-6183-6