Medical & Biotech – Page 84 – TechConnect Briefs

Integrated polymer-based microfluidic device for DNA preparation in pyrosquencer

Khuntontong P., Kua C.H., Gong M., Foo I.S.H., Goel S., Wang Z.P., Singapore Institute of Manufacturing Technology, SG

We present a polymer-based microfluidic device capable of performing various DNA preparation processes like enzymatic, end repairing, and purification. The system consisted of a disposable polymeric chip and a controlling unit. Mixing chambers, incubation chambers [...]

Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

Liu X., Lo R.C., Gomez F.A., California State University Long Beach, US

In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) [...]

Express Control of Toxicity and Content of Patulin by Optical Biosensors

Starodub N.F., Pilipenko I.V., Pilipenko L.N., Pilipenko I.V., Pilipenko L.N., Katsev A.M., National University of Life and Environmental Sciences of Ukraine, UA

In this report the experimental data about the determination of P toxicity by optical biosensors based on the application of chemiluminescence (ChL) of Daphnia magna living media (ChL-test) and bioluminescence (BL) of Ukrainian strains of [...]

A novel on chip analysis of dissolved Hg(II) in drinking water

Deshpande K., Mishra R.K., Pal S., Danielsson B., Willander M., Bhand S., Birla Institute of Technology & Science, Pilani-Goa Campus, IN

A novel biochip based detection for determination of ultra trace mercury (Ii), lead(II) and cadmium (II)in drinking water. High through put analysis, low sample volume, high sensitivity and rapid analysis is the hallmark of the [...]

Detection of aflatoxin B1 by SPR biosensor using fusion proteins as a linker

Ko S., Kim A.-R., Kim C.J., Kim A.-R., Kim C.J., Kwon D.Y., Korea Food Research Institute, KR

Because of the health risks of aflatoxin B1 (ATB1), it is essential to monitor the level of this mycotoxin in a variety of foods. HPLC and ELISA methods require skilled personnel, expensive equipments, and complex [...]

Innovative Nanoprobes for Multiparametric Magnetic Immunoassays: Elaboration and Characterization

Brioude A., de Beaucorps C., Alphandéry E., Benyettou F., Brioude A., Bonnin D., Weinmann P., Motte L., Lalatonne Y., Avicenne Hôspital, FR

Magnetic nanoparticles (MNPs) are commonly used for contrast enhancement in magnetic resonance imaging, as magnetic carriers in automated immunoassays instruments, or for cell concentration at the bench. They are being evaluated all over the world [...]

Biomedical Applications of Modified Carbon Glassy Electrode Sensor with Nanoparticles and Dendrimers

Leung S.W., Wang Y., Gu H., Lai J.C.K., Idaho State University, US

In our previous reports, we studied the development of biosensor platform that are capable of measuring biometabolites and environmental sensitive species, such as peroxide and nitrate/nitrate, to concentration in the order of ppb (parts per [...]

On-Chip Ion-selective Microesensor for Evaluation of Mitochondrial Membrane Potential

Lim T.-S., Dávila A., Wallace D.C., Burke P., UC Irvine, US

The mitochondrial membrane potential is used to generate and regulate energy in living systems, driving the conversion of ADP to ATP, and controlling apoptosis, all central to human health and disease. Therefore, there is a [...]

Development and practical application of biosensors based on the nanostructured silicon

Starodub N., Mel’nichenko N., Shmireva A., Shuljak L.N., National University of Life and Environmental Sciences of Ukraine, UA

In this report the principle new instrumental method of the biochemical diagnostics of the bovine leucosis, autoimmune process at the diabetes and the control mycotoxins through the registration of the formation of the specific immune [...]

Electrochemiluminescent sensor for clinical analysis based on Carbon Nanotube electrodes

Sanginario A., Demarchi D., Giorcelli M., Castellino M., Politecnico di Torino, IT

Presented application is based on the use of carbon nanotubes (CNTs) for biomolecular analysis using electrochemiluminescence (ECL) detection technique. For this purpose we have grown self standing cylinder-shaped blocks of Multi wall CNTs (MWCNTs) by [...]