In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 141 - 144
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topicss: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging