Medical & Biotech – Page 160 – TechConnect Briefs

Nano-Cantilevers for an Ultra-Sensitive Bio-Assay

Zheng J., Klein K., Pucha R., Sitaraman S.K., Sitaraman S.V., Merlin D., Rajalakshmi S., Sarma D.S.R., Gerwirtz A., Sitaraman S.K., Sitaraman S.V., Georgia Institute of Technology, US

Micro and Nano-scale cantilever arrays are being developed for an ultra sensitive bio-assay (Figure 1). The advantage of these cantilevers is that they eliminate the use of laser for deflection detection, which is impractical for [...]

New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules

Snabe T., Neves-Petersen M.T., Fojan P., Klitgaard S., Neves-Petersen M.T., Petersen S.B., Aalborg University, DK

Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized [...]

Surface Characterization of a Direct, Label-Free Piezoelectric Immunosensor Platform

Papadopoulou-Bouraoui A., Lejeune M., Barrero-Moreno J., Valsesia A., Manso M., Gilliland D., Ceccone G., Rossi F., European Commission - JRC, IT

During this study the QCM (Quartz Crystal Microbalance) crystal surface was functionalized in order to provide active amino groups, and serve as an active platform for antibody immobilization and the development of direct, label-free immunosensors. [...]

Nanobiodevices Integrating Biomolecular Motors

Ramachandran S., Wu D., Nitta T., Tucker R., Phillips A., Hess H., University of Washington, US

Biomolecular motors, such as the motor protein kinesin, can serve as force-generating modules in hybrid nanodevices. We will present recent progress in utilizing these motors for nanoscale transport of target reagents and discuss applications, such [...]

Bio Micro Sensors

Surface Modification Chemistries and their Utilization in Microarrays, Diagnostics and Drug Delivery

Lodhi M.A., Opperman G.W., Wall J.V., Anderson A.B., SurModics, Inc., US

Availability of DNA sequence data on numerous organisms has revolutionized the way biological queries are addressed today. Focus of activity has largely shifted from discovery to application. Things that were considered far-fetched only a few [...]

Design and Applications of a Novel Type of Hairpin Probes: Addressable Bipartite Molecular Hook (ABMH)

Landré J.B.P., Ruryk A., Schlicksbier T., Russwurm S., Deigner H.-P., University of East Anglia, UK

ABMHs but not TMBs display the characteristic melt curves obtained with MBs. Moreover, ABMHs specificity in solution (Figure 2) is similar to those of MBs (i.e. differentiation between a perfect match and a 1 mismatch [...]

A Demonstration of High-throughput Immunoassay and Small Molecule Binding on Protein Microarrays with SPR Microscopy

Kim G., Jiang L., Rathod P.K., Campbell C.T., Nishimoto A., Casasanta V., University of Washington, US

The use of Surface Plasmon Resonance (SPR) for the detection of biomolecular binding events at the nanometer scale is of great utility when expanded into microarray density formats. Protein microarray analysis is particularly well suited [...]

Apoptosis of the Human Neutrophils under a Lipopolisaccharide (LPS) of Proteus Mirabilis Condition Observed in Real Time by Atomic Force Microscopy (AFM)

Pleskova S.N., Guschina Yu., Zvonkova M.B., Medical State Academy Nizhniy Novgorod, RU

The goal of present work was to receive the images of apoptosis process in dynamics after addition of Proteus mirabilis 210 LPS and to measure the mechanical properties change of neutrophils’ membranes. We observed the [...]

New Ferrocene-Functionalized Cationic Polythiophene For Label-Free Electrochemical DNA Detection

Harding-Lepage P., Le Floch F., Leclerc M., Ho H.-A., Harding-Lepage P., Leclerc M., Université Laval, CA

A specific polycationic polythiophene was discovered in order to identify dsDNA from ssDNA. The main properties of this new polymer are its conformational possibilities associated with photophysics modifications. This conjugated polymer is still subject to [...]