Papers – Page 619 – TechConnect Briefs

Landscape Phage Probes for Breast Cancer Cells

Fagbohun O.A., Bedi D., Jayanna P.K., Deinnocentes P.A., Bird R.C., Petrenko V.A., Auburn University, US

Landscape phage libraries are collections of filamentous phages displaying random foreign peptides on all 4,000 surface domains of the major coat protein. They serve as a rich source of bioselective materials that are used in [...]

Landscape phage probes for PC3 prostate carcinoma cells

Jayanna P.K., Deinnocentes P., Bird R.C., Petrenko V.A., Auburn University, US

Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to [...]

Engineering hybrid nanostructures with phage particles

Makowski L., Chen L., Argonne National Laboratory, US

Assembling complex structures comprising multiple, inorganic functional elements represents a significant engineering challenge. We have constructed hybrid nanostructures based on T7 and M13 phage and incorporating a variety of metals and metal oxides. Synthesis of [...]

Engineering of bacteriophages displaying affinity tags on its head for biosensor applications

Tolba M., Brovko L.Y., Griffiths M.W., University of Guelph, CA

A method was developed for the introduction of affinity tags onto bacteriophage heads using a phage display technique. It was shown that T4 bacteriophage carrying a biotin-binding polypeptide (Biotin Carboxyl Carrier Protein, BCCP, from acetyl-CoA [...]

Landscape phage libraries as a source of bioselective nanomaterials

Kuzmicheva G.A., Sorokulova I.B., Jayanna P.K., Petrenko V.A., Auburn University, US

New generations of targeted diagnostic, therapeutic and imaging nanodevices require robust molecular recognition interfaces, which bind their targets with high specificity and selectivity. These bioselective interfaces can be formed by recombinant filamentous phages or their [...]

Modulating affinity of landscape phage nanoparticles by mutagenesis of the major coat protein

Kuzmicheva G.A., Eroshkin A.M., Potemkin V.A., Petrenko V.A., Auburn University, US

Phage peptide libraries proved to be invaluable source of the ligands specifically binding numerous analytes. Diversity and repertoir of the libraries are critical for the successful selection of ligands. In landscape libraries guest peptides are [...]

Targeted Drug-carrying Filamentous Phage Nanomedicines

Yacoby I., Bar H., Vax L., Benhar I., Tel Aviv University, IL

We present a novel application of filamentous bacteriophages as targeted drug carrying nanomedicines. The phages are engineered to display target-specificity-conferring peptides or proteins on their coat, and carry a large payload of a cytotoxic drug [...]

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