stem cells – TechConnect Briefs

A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair

Holmes B., Bulusu K., Plesniak M., Zhang L.G., Nanochon LLC, George Washington University, US

In recent years, 3D printing has become a popular and widely investigated method for the fabrication of large bone implants. 3D printed bone constructs, devices and treatments hold great potential to repair traumatic and chronic [...]

One and two dimensional inorganic nanoparticles’ interactions with fibroblasts and stem cells

Rashkow J.T., Talukdar Y., Lalwani G., Sitharaman B., Stony Brook University, US

We report the effects of one and two dimensional transition metal dichalcogenide nanoparticles, tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs), on fibroblastic cell (mouse fibroblasts (NIH-3T3) and human adipose derived stem cell (MSC)) [...]

Three-Dimensional Macroporous Carbon Nanotube Scaffolds for Stem Cell Expansion and Maintenance

Lalwani G., Gopalan A., D’Agati M., Patel S., Talukdar Y., Sitharaman B., Stony Brook University, US

Expansion of stem cells and maintenance of their self-renewal capacity in vitro requires specialized robust cell culture systems. Conventional approaches consisting of animal derived matrices and cocktail of growth factors have limitations such as consistency, [...]

Three-Dimensional Bioprinted Biomimetic Nanocomposite Scaffolds for Osteochondral Regeneration

Castro N.J., Zhang L.G., The George Washington University, US

The current work focused on the manufacture of a three-dimensional biphasic nanocomposite scaffold for osteochondral regeneration. Extrusion-based 3D printing in combination with ultraviolet light induced photocrosslinking allowed for good integration of two disparate polymeric materials [...]

PEG Incorporated Polymeric Microcapsules for Intramyocardial Delivery of Stem Cells Genetically Modified by Baculovirus

Paul A., Khan A.A., Abbasi S., Shum-Tim D., Prakash S., McGill University, CA

The ability of mesenchymal stem cells to self-renew and differentiate into specialized cell lineages makes them promising tools for regenerative medicine. Local injection and use of scaffolds had been employed earlier to deliver these cells; [...]

Synchrotron radiation microtomography for the ex-vivo and in-vivo evaluation of nanoparticle-labeled stem cell homing in muscular tissue

Fiori F., Farini A., Giuliani A., Manescu A., Villa C., Torrente Y., Polytechnic University of Marche, IT

This work was carried out in the frame of a more general research program aiming to use human stem cells to repair muscle damage in Duchenne Muscular Dystrophy. The results of X-ray microtomography experiments, carried [...]

Preparation of nanopatterned substrates via Dip-pen Nanolithography for stem cell applications

Oberhansl S., Lagunas A., Martinez E., Jamil H., Samitier J., Institute for Bioengineering of Catalonia, ES

Dip-pen Nanolithography is used to fabricate nanopatterned substrates for stem cell experiments. Factors which are known to play a role in stem cell differentiation are immobilized on these substrates. The highly controlled order, in which [...]

Attachment and Spreading of Human Embryonal Carcinoma Stem Cells on Nanosurfaces Using Optical Waveguides

Aref A., Horvath R., Ansari F., Ramsden J.J., Cranfield University, UK

Abstract Cell adhesion is an active process, carried out in vivo via receptor ligand-like interactions between cell surface adhesion molecules and the extracellular matrix. Initial cell surface reactions following contact may trigger multiple responses, which [...]

Behavior of Living Human Neural Networks on Microelectrode Array Support

Pizzi R., Gelain F., Fantasia A., Rossetti D., Vescovi A., University of Milan, IT

Researchers of the Department of Information Technologies of the University of Milano and of the Stem Cells Research Institute of the DIBIT-S. Raffaele Milano are experimenting the growth of human neural networks of stem cells [...]