Attachment and Spreading of Human Embryonal Carcinoma Stem Cells on Nanosurfaces Using Optical Waveguides

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Abstract Cell adhesion is an active process, carried out in vivo via receptor ligand-like interactions between cell surface adhesion molecules and the extracellular matrix. Initial cell surface reactions following contact may trigger multiple responses, which in turn result in either spreading or detachment of the cell. The set of adhesion and attachment molecules mediating the adhesive behaviour of stem cells and the kinetics of their interactions are as yet largely unknown. Substrate-stem cell investigations are important for therapeutic (such as 3D tissue growth) [1] and non therapeutic applications, e.g. cell development for therapy, disease modeling and protein production. Optical waveguide lightmode spectroscopy (OWLS) is a powerful technique for the precise quantifications of thin film material deposition on a solid waveguide surface [2] (within the sensing depth ~150 nm), and has also been used to measure the kinetics of cell adhesion and spreading on predefined surfaces [3], and the attachment of bacteria [4]. We have investigated the attachment and spreading kinetics of human carcinoma stem cells (TERA2.sp12) onto planer Si(Ti)O2 waveguides, covered with poly-L-lysine acting as substrates for the cells. Keywords: stem cells; optical waveguide; kinetics; attachment;spreading REFERENCES: 1. Griffith, L.G. Emerging design principles in biomaterials and scaffolds for tissue engineering. Ann. N.Y. Acad. Sci. 961 (2002) 83-95. 2. Ramsden, J.J. High resolution molecular microscopy. In: Proteins at Solid-Liquid Interfaces (ed. Ph. Dejardin), pp. 23–49. Heidelberg: Springer-Verlag (2006). 3. Ramsden, J.J., Li, S-Y., Heinzle, E. and Prenosil, J.E. Optical method for measurement of number and shape of attached cells in real time. Cytometry 19 (1995) 97-102. 4. Horvath, R. Henrik C. Pedersen, Nina Skivesen, David Selmeczi and Niels B. Larsen. Monitoring of living cell attachment and spreading using reverse symmetry waveguide sensing. Appl. Phys. Lett.86 (2005) 071101.

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Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 573 - 576
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topic: Chemical, Physical & Bio-Sensors
ISBN: 978-1-4200-8504-4