microfluidic – TechConnect Briefs

A stand-alone and portable microfluidic platform for quantitative multi-step assays

Parent C., Verplanck N., L. Achard J-, Boizot F., Charles R., Fouillet Y., CEA-LETI, FR

This paper reports an automated platform for quantitative multi-step assays composed of two main components: a microfluidic chip using collapsible chambers pneumatically actuated and a portable instrument to automate the fluidic operations. To achieve a [...]

Real-time bacterial capture and sorting using dielectrophoresis and electroosmosis

Simon S.X., Markewich H., Mallick E., Weber M., Fluid-Screen, Inc., US

Fluid-Screen, Inc. presents a bacterial capture and sorting method based on dielectrophoresis (DEP) and electroosmosis (EO). These electronic phenomena have been known to influence particle motion and capture since the 1960’s, however, technology based on [...]

Two-Dimensional Analysis of the Enhanced Magnetic Bioseparation in Microfluidic Systems

Shi Z., Sun J., Jia S., Zhang P., Xi'an Jiaotong University, CN

The separation of magnetically-labeled bioparticles in a microfluidic system is dependent on the characteristics of the particles and the carrier fluid as well as the external magnetic field. To discuss the influence of these factors, [...]

High efficiency capture of multiple bacterial species by microfluidic dielectrophoresis filter

Simon S., Weber M., Fluid-Screen, Inc., US

Fluid-Screen, Inc. presents a bacterial concentration and filtration method based on dielectrophoresis (DEP). DEP has been known to induce particle motion since the 1960’s. However, yields and reproducibility have consistently been low in practice, reducing [...]

On-line production of polyelectrolyte coating on monodisperse alginate beads in an integrated microfluidic cartridge

Laffite G., Forvi E., Hadji C., Authesserre C., Alessio M., Costa G., Boizot F., Rivera F., CEA-LETI, FR

Continuous flow microfluidic devices have appeared to be a promising technology to automatise Polyelectrolytes multilayers (PEMs) coating on microbeads with repeatable and reliable results. However, no microfluidic devices have demonstrated PEMs coating on hydrogel beads [...]

New microfluidic chip for the production of spherical gelled capsule for cell encapsulation

Dalle P., Morales S., Berthier J., Benhamou P-Y., Bellemin Comte A., Boizot F., Costa G., Rivera F., CEA-LETI, FR

Implantation of Langerhans islet in alginate shell is an alternative for the treatment of diabetes type 1. Finding an adapted process for cell encapsulation is a challenge. The process must product spherical and monodisperse capsules [...]

Rifampicin nanosuspension production using microfluidic device

Schianti J.N., Cerize N.N.P., Oliveira A.M., Derenzo S., Gongora-Rubio M.R., Polytechnic School - University of São Paulo, BR

In this work the results of a study of Rifampicin nanoprecipitation through antisolvent technique are presented, using microfluidic devices. Rifampicin is commonly used in Tuberculosis treatment and presents variability in bioavailability. The particle size and [...]

Multiplexed protein detection using antibody-conjugated microbead arrays assembled on a microfabricated electrophoretic device

Barbee K.D., Hsiao A.P., Roller E.E., Huang X., UCSD, US

We report the development of a microfabricated electrophoretic device for assembling high-density arrays of antibody-conjugated microbeads for chip-based protein detection. The device consists of a flow cell formed between a gold-coated silicon chip with an [...]

Transitions Between Epithelial and Mesenchymal States in Microfluidic Platform: Acquisition of Malignant and Stem Cell Traits

Aref A.R., Huang R.Y.J., Kang W., Jing S.W., Thiery J.-P., Kamm R.D., SMART, SG

Epithelial–mesenchymal transition (EMT) is an indispensable mechanism during morphogenesis, as without mesenchymal cells, tissues and organs will never be formed. Transitions between epithelial and mesenchymal states have crucial roles in embryonic development and without EMT, [...]

Vapor-assisted bonding of poly(dimethylsiloxane) and silicon-based substrates

Sofla A.Y.N., Martin C., University of Toronto, CA

We present a novel method to reliably bond PDMS to glass, Si, SiO2 and PDMS. In this vapor-assisted method the surface of the PDMS is first brought into contact with the substrate. The PDMS-substrate structure [...]