microarray – TechConnect Briefs

A Simple Method for Fabrication of Microarrays and Microfluidic Device Using PDMS Stamp

Lee H., Koh D., Oh K.W., SUNY at Buffalo, US

We describe a simple micro-fabrication method by using polydimethylsiloxane (PDMS) stamp on a glass substrate. While the microwells used previously facilitated the cell-to-cell formation by PDMS stamping method, not only should it be carefully controlled [...]

Wafer-scale photonic and plasmonic crystal sensors

George M.C., Liu J.-N., Farhang A., Williamson B., Black M., Wangensteen T., Fraser J., Petrova R., Cunningham B.T., Moxtek, US

This presentation will review recent results in 200 mm diameter wafer-scale fabrication, metrology, and optical modeling of various photonic and plasmonic crystal sensors, including 1D photonic crystals based on leaky-waveguide mode resonance, as well as [...]

A Simple Method for Fabrication of Microarrays and Microfluidic Device Using PDMS Stamp

Lee H., Koh D., Oh K.W., SUNY at Buffalo, US

We describe a simple micro-fabrication method by using polydimethylsiloxane (PDMS) stamp on a glass substrate. While the microwells used previously facilitated the cell-to-cell formation by PDMS stamping method, not only should it be carefully controlled [...]

Novel three-dimensional porous film for single-cell microarray applications

Ranjan S., Yong Z., National University of Singapore, SG

Cell-based studies yield data averaged over a large number of cells which overlook the rich information that can possibly be obtained when cells are studied individually. For this reason, much emphasis has been placed over [...]

High-throughput multiplex protein analysis for limited sample size on a single slide

Rozhok S., Ouyang K., Dixon D., Nelson M., NanoInk Inc, US

To meet the challenges of new genomic and proteomic applications, rapid and sensitive analysis of large numbers of interactions in parallel will be required. High-density microarrays are ideally suited for parallel multiplex screening of thousands [...]

Size Matters: The outer diameter of multiwalled carbon nanotubes (MWCNTs) affects gene expression in female fathead minnows (Pimephales promelas)

Carter B.J., Griffitt R.J., Brown-Peterson N., Hammers H.R., Barber D.S., EcoArray, Inc., US

We used oligonucleotide microarrays to examine the gene expression patterns in female fathead minnows (Pimephales promelas) exposed to two different sizes (<8 nm and 50-80 nm outer diameter) and three concentrations (0.1 mg/L, 0.3 mg/L, [...]

Hollow Atomic Force Microscopy Probes for Nanoscale Dispensing of Liquids

Meister A., Przybylska J., Niedermann P., Santschi C., Heinzelmann H., CSEM SA, CH

The demand for specific tools intended for the deposition of small amount of material on a nanoscale at predefined location is continuously increasing. Several dispensing tools for liquid, such as ink-jet or pin-spotting heads have [...]

Homogeneous Bioluminescence Resonant Energy Transfer On High Density Array Imaging Chip

Filanoski B., Rastogi S., Cameron E., Mishra N., Maki W., University of Idaho, US

Fluorescent microarray technologies are widely used in molecular biology for studying gene expression, immune detection and drug discovery. Fluorescent assays are very sensitive but present problems with many bio-agents having inherent fluorescence which interfere with [...]

Fast and Efficient Nucleic Acid Testing by ST‘s In-CheckTM Lab-on-Chip Platform

Conoci S., Di Francesco F., Di Pietro P., Di Trapani G., Petralia S., Amore M.G., San Biagio F., Alaimo G., Iacono G., Alessi E., Ricceri D., Di Francesco F., Di Pietro P., Di Trapani G., Di Francesco F., Di Pietro P., Di Trapani G., Palmieri M., STMicroelectronics, IT

Nucleic Acid Testing (NAT) represents one the most sensitive and effective method for several applications in the molecular diagnostic area, including clinical, industrial, veterinary and research applications. In this context, the development of miniaturized devices [...]

Novel Photonic Technique Creates Micrometer Resolution Multi-sensor Arrays and Provides a New Approach to Coupling of Genes, Nucleic Acids, Peptide Hormones and Drugs to Nanoparticle Carriers

Neves-Petersen M.T., Duroux L., Duroux M., Duroux L., Duroux M., Skovsen E., Neves-Petersen M.T., Petersen S.B., Professor, DK

Photonic induced immobilisation is a novel patented technology resulting in spatially oriented and localised covalent coupling of biomolecules onto surfaces. Immobilisation has been achieved for a wide selection of proteins, e.g., human plasminogen, alkaline phosphatase, [...]