lipid bilayer – TechConnect Briefs

Investigations on Transmembrane Ion Channels Suspended over Porous Silicon Membranes

Tantawi K.H., Berdiev B., Cerro R., Williams J.D., University of Alabama in Huntsville, US

This article presents a batch-fabricated porous silicon membrane tool used to investigate transmembrane protein functionality in complex biological systems. We address the physical and chemical requirements fabricate the thin porous membrane, attached a lipid bilayer, [...]

Microfluidic Platforms with Micro- and Nanopore Membranes and in situ Formation of Lipid Bilayer in the Microfluidic Platform

Choi J., Kim N., Park S., Louisiana State University, US

Artificial lipid bilayer membranes have come into the spotlight as topics of fundamental properties of biomaterials, targets for drug discovery and safety screening, and analytic platforms for characterization and orientation discrimination of polynucleotide molecules. Lipid [...]

Highly passivated silicon chips with micron aperture for the pA measurements of single ion channels

Varnier A., Plénat T., Ghenim L., Marcel-Kermarrec F., Picollet-d’Hahan N., Agache V., Sauter F., Chatelain F., Fuchs A., CEA-Grenoble, FR

Detection of single channel activities on a high throughput device is of major interest for pharmaceutical industries developing new drugs targeting ion channels. A silicon chip, with 9 silicon membranes having a micron aperture each, [...]

Integrated Techniques for Transmembrane Protein Sensing

Wilk S.J., Petrossian L., Goryll M., Thornton T.J., Goodnick S.M., Tang J.M., Eisenberg R.S., Arizona State University, US

We present a method for the microfabrication of apertures in a silicon substrate using well-known cleanroom technologies and a novel approach using phase sensitive detection to measure single channels of transmembrane proteins. These proteins are [...]