label-free – TechConnect Briefs

Discovering of Collagen-1’s Role in Producing Superconducting Current in Nanobiomimetic Superlattice Structured Organometallic Devices at Room Temperature Enabled Direct Quantitation of Sub pg/mL Collagen-1 by a Voltage and a Cyclic Voltammetry Method

Chen E.T., Thornton J.T., Kissinger P.T., Duh S-H, Advanced Biomimetic Sensors, Inc., US

Cooper pair electrons producing superconducting current in near zero Kelvin temperature at the Josephson-junction in the superconductors show its potential utilities. Could also a superconducting current produced by a superlattice organometallic device enable direct detection [...]

Nanobiomimetic Superlattice Superconductive/Memristive Organometallic Devices at Room Temperature Serve as Chronoamperometric Sensors for sub fg/mL Collagen-1 Detection in Human Serum

Chen E.T., Thornton J.T., Kissinger P.T., Duh S-H, Advanced Biomimetic Sensors, Inc., US

Superconductive/Memristive devices have potential applications in supercomputing and in health industry was proposed in literature. Clinically detection range at low concentration for collagen-1 is difficult to accomplish due to the denaturing of protein Matrix Matelloprotinase-2 [...]

Organic Nanobiomimetic Memristor/Memcapacitor Devices Ultrasensitive Direct Detect Matrix Matelloproteinase-2 in Human Serum

Chen E.T., Thornton J., Duh S-H, Advanced Biomimetic Sensors, US

Matrix Matelloproteinase-2 (MMP-2) plays a key role in many diseases. A new type of dual-functioning device was developed for fast, direct ultrasensitive detection of MMP-2 using NIST SRM965A reference human serum specimens under label-free, probe-free [...]

Functionalized Nanopipette: towards single cell immunoassay

Actis P., Jejelowo O., Pourmand N., UC Santa Cruz, US

Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies [...]

Microfluidic Devices for Rapid Separation and Sensing of Cells

Bose S., Lee C.H., Karp J.M., Karnik R., Massachusetts Institute of Technology, US

We report a new method to separate cells in microfluidic devices by directing the trajectories of cells along asymmetric receptor edges. This approach relies on cell rolling, a physiological phenomenon involving transient interactions of the [...]

Label-free direct detection of analytes from optically inaccessible samples

Brousseau L.C., Quantum Logic Devices, US

Fluorescent optical methods for biomolecular detection are widely used for research and diagnostic applications. However, these techniques are useful only in sample matrices that are optically transparent non-scattering, which precludes most biologically relevant fluids, such [...]

Real-time single-molecule dynamics observed with a nanoscale transistor

Brousseau L.C., Quantum Logic Devices, US

Direct observation of single molecular reactions and motions are impossible by optical techniques due to diffraction limits. However, ensemble measurements of binding dynamics in biological systems offer limited understanding of individual actors in metabolic and [...]

‘Lab on a chip’ Label Free Protein Sensor Systems Based on Polystyrene Bead and Nanofibrous Solid Supports

Kunduru V., Prasad S., Patra P.K., Sengupta S., Portland State University, US

Polystyrene bead and electrospun polystyrene nanofibers are employed as subjects of study in this protein biomarker sensor. The study is conducted to compare the sensitivity and efficiency of the polystyrene nanofibers and microspheres as protein [...]

Label-free and Real-Time Photonic Band-Gap Silica Nanosensor

Chyan J.Y., Yang S.C., Yeh J.A., National Tsing Hua University, TW

A photonic band-gap nanosensor based on porous silica is proposed in the study. The photonic band-gap silica nanosensor is easy to fabricate and simple to use. Its optical characteristics can be easily modulated by electrochemical [...]

Au Nanoparticle Coupled- Surface Plasmon Resonance Immuno-sensor for Sensitivity Enhancement

Kim H-S, Kim J.H., Kim H-S, Kim J.H., Ko S-H., Cho Y-J, Kim H-S, Kim J.H., Ajou University, KR

In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. Although the nanoparticle conjugated method contributes to the enhancement [...]