Chen E.T., Thornton J.T., Kissinger P.T., Duh S-H
Advanced Biomimetic Sensors, Inc., US
Keywords: energy harvesting, Josephson junction, label-free, nanobiomimetic organometallic superconductor, probe-free, sensing device, superlattice quantum membrane
Cooper pair electrons producing superconducting current in near zero Kelvin temperature at the Josephson-junction in the superconductors show its potential utilities. Could also a superconducting current produced by a superlattice organometallic device enable direct detection of collagen, without denaturing of a nature enzyme? Clinically useful detection range at low end for collagen-1 is difficult to accomplish due to denaturing protein Matrix Matelloprotinase-2 (MMP-2). Two types of superlattice superconductive quantum devices were developed. Sensor 1 mimicked the innate MMP-2 with cystein attached, and Sensor 2 mimicked the denatured MMP-2 by knockout of the cystein group. Sensor 1 and 2 were made by cross-linking multiple quantum organometallic material on gold chips forming superlattice by self-assembling. Sensor 3 used the same polymers linked with the MMP-2 protein without metal ions applied. Quantitation of collagen-1 without antibody, no labeling and no tracers was used. Our results shown Sensor 1 produced superconducting current at room temperature, that is exponentially proportional to collagen concentration in an innate state using NIST SRM 965A serums by the cyclic voltammetry method; Denatured Sensor 1 produced a 10-fold higher superconductivity compared with Sensor 2 in the presence of 50 ng/mL collagen-1 in a cyclodextrin media; Results obtained by the voltage method shown Sensor 1 produced the action potential inversely proportional to the collagen concentration over a linear range 0.5 pg/mL-200 ng/mL in PBS vs. NIST serum (n=18 vs. n=15) with a pooled relative standard deviation of 2.0% vs. 0.3%, respectively. The recovery results from the measured collagen in PBS media vs. human serum (n=15) have an agreement of 92% with a s.d. of 0.03% over the studied range. Without denaturing, collagen-1 communicated to Sensor 3 using the CV method at 300Hz from 0.5 pg/mL to 0.5 ng/mL in PBS media. Our accomplishments are based on our prior works: Nanobiomimetic Memristor/memcapacitors’ Function as a Voltage Sensor for Direct and Reagent-free Detection of sub pg Lipopolysaccharide (LPS) in Different Types of Milks for Infants, Sensors, Diagnostics & Imaging, TechConnect Briefs, 4, 140-143, 2016. Organic Nanobiomimetic Memristive/Memcapacitive Devices Ultrasensitive Direct Detect Matrix Matelloproteinase-2 in Human Serum, Biotech, Biomaterials and Biomedical, TechConnect Briefs, 271-274, 2017. Partially listed patents: Nanopore array structured devices for biosensing and energy storage, US 8,641,876, 2014. Nanobiomimetic Supercapacitors with High Rate High Energy Storage, US 9,443,665, September, 2016. Nanostructured biomimetic device for detecting a cancer cell or cancer cells US 9,534,999, Jan. 2017. The new accomplishments are:1) Revolutionarily changed the denaturing protein approach that used for centuries and reduced the assay burden and orders of magnitude increased the detection sensitivity compared literature; 2) Our approach is innovative by utilizing collagen’s unique role in superconducting for the first time; 3) Reagent-free, label-free and probe-free advantages reduced contamination ;4) simple and portable. It will open wide market applications. 5). The robust performance of the superconductor devices has provided a foundation for further development toward automation.
Journal: TechConnect Briefs
Volume: 4, Informatics, Electronics and Microsystems: TechConnect Briefs 2018
Published: May 13, 2018
Pages: 43 - 46
Industry sector: Sensors, MEMS, Electronics
Topics: Advanced Manufacturing, Nanoelectronics
ISBN: 978-0-9988782-1-8