biotin – TechConnect Briefs

Aerosol manufacturing of plasmonic biosensors with silica coated nanosilver

Sotiriou G.A., Blattmann C.O., Pratsinis S.E., ETH Zürich, CH

Development of biosensors for medical applications is an on-going research field of great importance. In particular localized surface plasmon resonance (LSPR) biosensors show a high potential to detect analytes at low concentrations in biological fluids. [...]

Attenuated-Affinity Biotin Analogs for Catch-and-Release with Streptavidin

Corry S., Ying L-Q, Branchaud B., Life Technologies, US

The binding between streptavin and biotin, which is one of the strongest non-covalent interactions known in nature, is used ubiquitously in molecular biology. However, when a biotinylated molecule is captured for testing purposes and cannot [...]

Surface Plasmon Spectral Shifts of Functionalized Gold Nano particles for the Use in Biosensors

Chatterjee S., Moriarty G., Gersten B., Queens College, CUNY, US

Recently there has been interest in the use of gold nanoparticles as a colorimetric method to detect the hybridization event of DNA [1-4]. The method uses the physical phenomena that nanoparticles of gold exhibits the [...]

A highly sensitive fluorescent immunoassay based on avidin labeled nanocrystals

Sin K-K, Chan C.P-Y, Pang T-H, Seydack M., Renneberg R., the Hong Kong University of Science and Technology, HK

Nanocrystals of the fluorogenic precursor fluorescein diacetate (FDA) were applied as labels to enhance assay sensitivity in our previous studies. Each FDA nanocrystal can be converted into ~2.6 x 106 fluorescein molecules, which is useful [...]

Detection of Small Molecule-Protein Binding with Amorphous Silicon Nanostructures

Lund J., Mehta R., Parviz B., University of Washington, US

We present the fabrication and characterization of a nano-scale sensor made of amorphous silicon (a-Si) for electronic detection of small molecule –protein binding. The main component of the detector is a nano-scale a-Si semicircle with [...]

Effect of Length of Biotin Modified n-Alkanethiolates on the Formation of a Self-Assembled Streptavidin-Biotin Monolayer on a Gold Surface

Kim D.-J., Cho E.C., Koo K.-K., Sogang University, KR

The molecular recognition between streptavidin and biotin makes the streptavidin-biotin system very useful in a wide range of biotechnological applications. Currently, biotin-modified n-alkanethiolates have been extensively studied to create a wide variety of self-assembled monolayers [...]

MEMS for Drug Screening Applications

Zhou J., Zurn S., Markus D., Mantell S.C., Polla D., Smith G., University of Minnesota, US

MEMs offer a quick, low cost method for screening new drugs. In this MEMs application, microbeams were designed such that an affinity between the beam surface (coated with a biomolecule) and a second biomolecule (simulating [...]