US – Page 202 – TechConnect Briefs

A Novel Microscale Coulter Counter for Cell Monitoring and Detection

Wu Y., Critser J., Almasri M., University of Missouri, US

A MEMS based Coulter counter for cell detection and monitoring is designed, fabricated and characterized. The novelty of this design is the use of mixing, focusing and detection on a single chip as well as [...]

Counting and Sizing of Virus and Protein Aggregates by Nanoparticle Tracking Analysis (NTA)

Griffiths D., Hole P., Smith J., Malloy A., Carr B., NanoSight, US

A new analytical system is described which provides total particle count and high-resolution size measurements for biological structures such as virus and protein aggregates. This system builds on the Nanoparticle Tracking and Analysis (NTA) technique [...]

Nanoparticle size and shape evaluation using the TSOM optical microscopy method

Attota R., Silver R.M., Vladar A., NIST, US

We present a novel optical technique that produces nanometer dimensional measurement sensitivity using a conventional optical microscope, by analyzing through-focus scanning optical microscope (TSOM - pronounced as ‘tee-som’) images obtained at different focus positions. In [...]

Biological robotics and nanorobot red cells: Characterization and applications

Sharma A., Sharma R., Sharma A., Sharma R., Chen C-J., TCC/Florida State University, US

In human body, nanorobot-like red blood cells get exposed to human body fluids and many physico-chemical changes inside while these nanorobots (fullerene structure coated hemoglobin embedded iron inorganic elements) remain safe and perform killer action [...]

Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

Liu X., Lo R.C., Gomez F.A., California State University Long Beach, US

In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) [...]

Biomedical Applications of Modified Carbon Glassy Electrode Sensor with Nanoparticles and Dendrimers

Leung S.W., Wang Y., Gu H., Lai J.C.K., Idaho State University, US

In our previous reports, we studied the development of biosensor platform that are capable of measuring biometabolites and environmental sensitive species, such as peroxide and nitrate/nitrate, to concentration in the order of ppb (parts per [...]

On-Chip Ion-selective Microesensor for Evaluation of Mitochondrial Membrane Potential

Lim T.-S., Dávila A., Wallace D.C., Burke P., UC Irvine, US

The mitochondrial membrane potential is used to generate and regulate energy in living systems, driving the conversion of ADP to ATP, and controlling apoptosis, all central to human health and disease. Therefore, there is a [...]

Non-labeled DNA Biosensor Post-Processing Signals Analyses

Afuwape S.A., National University, US

The evolution of DNA biosensors should match commercial emergency of Nanotechnology in this genomic era. The ubiquitous oligonucleotide microarray ‘lab–on-chip’ is limited by high cost and man-power. However, emerging portable non-label DNA biosensors are yet [...]

Nanospring™ Enhanced Enzyme-linked Immunosorbent Assay for Increased Sensitivity and Early Detection

Beaux M.F., Cantrell T., Prakash T., Corti G., McIlroy D.N., Norton M.G., GoNano Technologies, Inc., US

GoNano Technologies NanospringTM material is proposed as an enhancement of enzyme-linked immunosorbent assay (ELISA) to increase detection sensitivity. The high surface area and hydroxyl rich surface chemistry provides greater attachment capability and decreased functional surface [...]

Functionalized Nanopipette: towards single cell immunoassay

Actis P., Jejelowo O., Pourmand N., UC Santa Cruz, US

Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies [...]