DNA microarrays are usually constructed using photolithographic synthesis of oligonucleotide probes or by spotting of pre-synthesized probes. These are expensive methods, and only one sample can be applied on a microarray chip at one time. [...]
Many emerging biological and medical applications involve the development of protein arrays at nanoscale.1 Dip-pen lithography2 nanografting and conducting tip AFM writing are capable of creating nano-protein patterns with 50-100 nm resolution over small surface [...]
, Hiddessen A.L.
, Wu K.J.J.
, Christian A.T.
, Dugan L.C.
, Stone G.
, Camarero J.
, Hinz A.K.
, Hubbell J.A.
, Lawrence Livermore National Laboratory, US
We have developed a novel method for patterning surface chemistry: Photocatalytic Lithography. This technique relies on inexpensive stamp materials and light; it does not necessitate mass transport or specified substrates, and the wavelength of light [...]
On microarrays the transport of the target molecules towards the spots only occurs by molecular diffusion. As a result, microarray analyses require overnight incubation and are highly inefficient in terms of capture rate and detection [...]
Several solid phase microarray based methods for the detection of the genomic materials have been developed based on amplifying the target molecules. Since proteins cannot be amplified, a similar approach to protein detection faces some [...]
Self-assembly of gold nanoparticles on e-beam nano-patterns towards protein nanoarray. A gold colloid suspensions containing different size nanoparticles, will be dumped in succesion, and the particles will self assemble into proper sized trenches.
For the past decade, microarray technology has evolved from genomics to proteomics. This trend offers several advantages. In small volumes, biochemical reactions may not be diffusion-limited and may thus be more efficient; less reagent and [...]
We make a mixed Self Assembly Monolayer for improve binding efficiency antibody, this method provide s stereo advantage and didn't need to modify antibody by moelcuar biology ono by one.
Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent [...]
In this paper, we introduce a new application of modeling tool; Boolean Networks (BN). In particular, it shows how, by means of a Boolean Networks (BN), the protein production process can be modeled and analyzed. [...]
In order to respond to new demands and needs in modern medical diagnostics and biomedical research applications, a lot of new DNA detection approaches have been reported. However, most of these methods have the disadvantage [...]
A specific polycationic polythiophene was discovered in order to identify dsDNA from ssDNA. The main properties of this new polymer are its conformational possibilities associated with photophysics modifications. This conjugated polymer is still subject to [...]
The goal of present work was to receive the images of apoptosis process in dynamics after addition of Proteus mirabilis 210 LPS and to measure the mechanical properties change of neutrophils’ membranes. We observed the [...]
The use of Surface Plasmon Resonance (SPR) for the detection of biomolecular binding events at the nanometer scale is of great utility when expanded into microarray density formats. Protein microarray analysis is particularly well suited [...]
ABMHs but not TMBs display the characteristic melt curves obtained with MBs. Moreover, ABMHs specificity in solution (Figure 2) is similar to those of MBs (i.e. differentiation between a perfect match and a 1 mismatch [...]
Availability of DNA sequence data on numerous organisms has revolutionized the way biological queries are addressed today. Focus of activity has largely shifted from discovery to application. Things that were considered far-fetched only a few [...]
Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: May 8, 2005
Industry sector: Medical & Biotech