DNA, Protein, Cells and Tissue Arrays

Fast DNA Hybridization on a Multi-Sample Multi-probe Microfluidic Microarray Compact Disc

Peng X.Y., Li P.C.H., Yu H-Z, Parameswaran A.M., Beardmore S., Chou W.L., Simon Fraser University, CA

DNA microarrays are usually constructed using photolithographic synthesis of oligonucleotide probes or by spotting of pre-synthesized probes. These are expensive methods, and only one sample can be applied on a microarray chip at one time. [...]

The Large Scale Fabrication of Protein Nanoarrays Based on Nanosphere Lithography

Cai Y., Ocko B.M., Brookhaven National Laboratory, US

Many emerging biological and medical applications involve the development of protein arrays at nanoscale.1 Dip-pen lithography2 nanografting and conducting tip AFM writing are capable of creating nano-protein patterns with 50-100 nm resolution over small surface [...]

Biomolecular Patterning via Photocatalytic Lithography

Bearinger J.P., Hiddessen A.L., Wu K.J.J., Christian A.T., Dugan L.C., Stone G., Camarero J., Hinz A.K., Hubbell J.A., Lawrence Livermore National Laboratory, US

We have developed a novel method for patterning surface chemistry: Photocatalytic Lithography. This technique relies on inexpensive stamp materials and light; it does not necessitate mass transport or specified substrates, and the wavelength of light [...]

Enhancement of DNA Microarray Hybridization using Shear-Driven Microfluidics

Vanderhoeven J., Pappaert K., Dutta B., Vanhummelen P., Baron G.V., Desmet G., Free University of Brussels, BE

On microarrays the transport of the target molecules towards the spots only occurs by molecular diffusion. As a result, microarray analyses require overnight incubation and are highly inefficient in terms of capture rate and detection [...]

Ultramicro Protein Arrays for Cytokine Detection

Radke K., Ryckman K., Nettikadan S., Lynch M., Mosher C., Xu J., Henderson E., BioForce Nanosciences, Inc., US

Several solid phase microarray based methods for the detection of the genomic materials have been developed based on amplifying the target molecules. Since proteins cannot be amplified, a similar approach to protein detection faces some [...]

Self-Assembly of Gold Nanoparticles on E-Beam Nano-Patterns Towards Protein Nanoarray

Powell T., Yoon J-Y, University of Arizona, US

Self-assembly of gold nanoparticles on e-beam nano-patterns towards protein nanoarray. A gold colloid suspensions containing different size nanoparticles, will be dumped in succesion, and the particles will self assemble into proper sized trenches.

Protein Array Fabricated by Micro-Contact Printing Method for Detection of Marker Protein to Genetically Modified Organisms

Lee W., Paek S-H, Choi J.W., Sogang Univerisity, KR

For the past decade, microarray technology has evolved from genomics to proteomics. This trend offers several advantages. In small volumes, biochemical reactions may not be diffusion-limited and may thus be more efficient; less reagent and [...]

Binding Efficiency Improvement of Antibody to Antigen on Mixed Self-Assembly Monolayers

Wu C-L, Lin Y-W, Hsu Y-L, Tseng F-G, Chieng C-C, National Tsing Hua University, TW

We make a mixed Self Assembly Monolayer for improve binding efficiency antibody, this method provide s stereo advantage and didn't need to modify antibody by moelcuar biology ono by one.

Imaging Ellipsometry: A Powerful Method for Biochip Development, QC and Evaluation

Laging M., Hoenig D., Eing A., Accurion, US

Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent [...]

Boolean Networks (BN) Based Molecular Biology Modeling: Protein Process

Yeol J.W., Barjis I., Barjis J., Barjis I., Barjis J., Berri S., Ryu Y., Polytechnic University, US

In this paper, we introduce a new application of modeling tool; Boolean Networks (BN). In particular, it shows how, by means of a Boolean Networks (BN), the protein production process can be modeled and analyzed. [...]

Cationic Polythiophene for Label-Free, Colorimetric and Fluorometric Detection of DNA

Ho H.-A., Leclerc M., Université Laval, CA

In order to respond to new demands and needs in modern medical diagnostics and biomedical research applications, a lot of new DNA detection approaches have been reported. However, most of these methods have the disadvantage [...]

New Ferrocene-Functionalized Cationic Polythiophene For Label-Free Electrochemical DNA Detection

Harding-Lepage P., Le Floch F., Leclerc M., Ho H.-A., Harding-Lepage P., Leclerc M., Université Laval, CA

A specific polycationic polythiophene was discovered in order to identify dsDNA from ssDNA. The main properties of this new polymer are its conformational possibilities associated with photophysics modifications. This conjugated polymer is still subject to [...]

Apoptosis of the Human Neutrophils under a Lipopolisaccharide (LPS) of Proteus Mirabilis Condition Observed in Real Time by Atomic Force Microscopy (AFM)

Pleskova S.N., Guschina Yu., Zvonkova M.B., Medical State Academy Nizhniy Novgorod, RU

The goal of present work was to receive the images of apoptosis process in dynamics after addition of Proteus mirabilis 210 LPS and to measure the mechanical properties change of neutrophils’ membranes. We observed the [...]