Papers:
A flow-assisted point-of-care testing device based on gravitational field-flow fractionation for analysis on biological fluids
Mirasoli M., Cevenini L., Casolari S., Roda A., Roda B., Di Carlo A., Reschiglian P., Roda A., Roda B., University of Bologna, IT
The “Point-Of-Care Testing” (POCT) approach is based on the development of portable analytical platforms suitable to perform analysis directly in any required place. A POCT device should combine portability, minimum sample pre-treatment, and the possibility [...]
Nanoparticle size and shape evaluation using the TSOM optical microscopy method
We present a novel optical technique that produces nanometer dimensional measurement sensitivity using a conventional optical microscope, by analyzing through-focus scanning optical microscope (TSOM - pronounced as ‘tee-som’) images obtained at different focus positions. In [...]
Counting and Sizing of Virus and Protein Aggregates by Nanoparticle Tracking Analysis (NTA)
A new analytical system is described which provides total particle count and high-resolution size measurements for biological structures such as virus and protein aggregates. This system builds on the Nanoparticle Tracking and Analysis (NTA) technique [...]
Hollow-fiber flow field-flow fractionation: a novel pre-MS method for proteomics
It is well acknowledged that both number and dynamic range of the proteins present in biological systems lead to the need of a “simplified” and more efficient way to proteome analysis. The availability of so-called [...]
Analysis and characterization of multifunctional oligothiophene-silica hybrid nanomaterials by AF4-MALS-Fl
Rambaldi D.C., Zattoni A., Reschiglian P., Melucci M., Zambianchi M., Barbarella G., Bonacchi S., Montalti M., Rampazzo E., University of Bologna, IT
We present results indicating that Asymmetrical Flow Field-Flow Fractionation (AF4) on-line coupled with Multi-Angle Light Scattering (MALS) and Fluorescence (FD) detection can become the unparallel methodology for morphological and optical characterization of new, structured, fluorescent [...]
A Novel Microscale Coulter Counter for Cell Monitoring and Detection
A MEMS based Coulter counter for cell detection and monitoring is designed, fabricated and characterized. The novelty of this design is the use of mixing, focusing and detection on a single chip as well as [...]
A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis
Lattuada D., Roda B., Pignatari C., Magni R., Cattaneo A., Bolis G., Reschiglian P., University of Bologna, IT
HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]
A novel stem cell tag-less sorting method
Roda B., Lanzoni G., Alviano F., Zattoni A., Costa R., Di Carlo A., Marchionni C., Franchina M., Ricci F., Tazzari P., Pagliaro P., Scalinci S.Z., Bonsi L., Reschiglian P., Bagnara G.P., University of Bologna, IT
New methods for stem cells isolation are being developed. Common procedures for stem cell sorting involve flow cytometry which makes use of fluorescent or magnetic tags to isolate and separate cells which are phenotypically different. [...]
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Biomaterials, Materials Characterization & Imaging
ISBN: 978-1-4398-3415-2