A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis

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HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]

Analysis and characterization of multifunctional oligothiophene-silica hybrid nanomaterials by AF4-MALS-Fl

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We present results indicating that Asymmetrical Flow Field-Flow Fractionation (AF4) on-line coupled with Multi-Angle Light Scattering (MALS) and Fluorescence (FD) detection can become the unparallel methodology for morphological and optical characterization of new, structured, fluorescent [...]

A flow-assisted point-of-care testing device based on gravitational field-flow fractionation for analysis on biological fluids

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The “Point-Of-Care Testing” (POCT) approach is based on the development of portable analytical platforms suitable to perform analysis directly in any required place. A POCT device should combine portability, minimum sample pre-treatment, and the possibility [...]