University of Bologna – TechConnect Briefs

A novel stem cell tag-less sorting method

Roda B., Lanzoni G., Alviano F., Zattoni A., Costa R., Di Carlo A., Marchionni C., Franchina M., Ricci F., Tazzari P., Pagliaro P., Scalinci S.Z., Bonsi L., Reschiglian P., Bagnara G.P., University of Bologna, IT

New methods for stem cells isolation are being developed. Common procedures for stem cell sorting involve flow cytometry which makes use of fluorescent or magnetic tags to isolate and separate cells which are phenotypically different. [...]

A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis

Lattuada D., Roda B., Pignatari C., Magni R., Cattaneo A., Bolis G., Reschiglian P., University of Bologna, IT

HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]

Analysis and characterization of multifunctional oligothiophene-silica hybrid nanomaterials by AF4-MALS-Fl

Rambaldi D.C., Zattoni A., Reschiglian P., Melucci M., Zambianchi M., Barbarella G., Bonacchi S., Montalti M., Rampazzo E., University of Bologna, IT

We present results indicating that Asymmetrical Flow Field-Flow Fractionation (AF4) on-line coupled with Multi-Angle Light Scattering (MALS) and Fluorescence (FD) detection can become the unparallel methodology for morphological and optical characterization of new, structured, fluorescent [...]

Hollow-fiber flow field-flow fractionation: a novel pre-MS method for proteomics

Rambaldi D., Zattoni A., Reschiglian P., Johann C., University of Bologna, IT

It is well acknowledged that both number and dynamic range of the proteins present in biological systems lead to the need of a “simplified” and more efficient way to proteome analysis. The availability of so-called [...]

A flow-assisted point-of-care testing device based on gravitational field-flow fractionation for analysis on biological fluids

Mirasoli M., Cevenini L., Casolari S., Roda A., Roda B., Di Carlo A., Reschiglian P., Roda A., Roda B., University of Bologna, IT

The “Point-Of-Care Testing” (POCT) approach is based on the development of portable analytical platforms suitable to perform analysis directly in any required place. A POCT device should combine portability, minimum sample pre-treatment, and the possibility [...]

Breakdown in the Output Characteristics of Deep Submicron, a-Si:H TFTs

Colalongo L., Valdinoci M., Forunato G., Mariucci L., Rudan M., University of Bologna, IT

Purpose of this work is to investigate the breakdown in the output characteristics of short-channel (0.2-1.o ,um) amorphous silicon (a-Si:H) thin-film transistors (TFTs). Such effect which, in the case of a-Si:H devices, has been detected [...]