Papers – Page 50 – TechConnect Briefs

Quantum dot (Qdot) labeling of gene expression analysis and single molecule imaging in neurons

Jeromin A., Riley Z., Byrnes E., Parry S., Smith S., Dee N., Jones A., Allen Institute for Brain Science, US

The Allen Brain Atlas1 is the first large scale atlas of gene expression in the mouse brain, using chromogenic in situ hybridization (cISH) of over 20,000 genes.Qdots have excellent potential for muliplex imaging of gene [...]

CNT and Organic FETs Based Two-Way Transducing of the Neurosignals

Sklyar R., Independent researcher, UA

A superconducting field-effect transistor (SuFET) based neurotransducer (sensor) with carbon nanotubes (CNT) or pickup coil kind of input circuit for the nerve and neuron impulse has been designed. Electronic or ionic currents in conductors or [...]

Advances in Automated 3D cortex image data acquisition

Lich B., Greiser J., Faber P., Knott G., FEI Company, NL

The study of the 3D architecture of cortex requires the creation of image datasets with sufficient detail in all directions. The detail level required to produce meaningful data requires imaging quality that allows identification of [...]

Evaluation For The Current Management of Pompe Disease

Khan U.A., University of Manchester, UK

Pompe disease in an autosomal recessive inherited condition characterised by deficiency of the enzyme acid -glucosidase. This causes accumulation of glycogen in muscles, significantly in the heart. The affect is a progressive muscle dysfunction, leading [...]

Evolutionary selection of bone mineral hydroxyapatite binding peptide using landscape phage library

Modali S., Abbineni G., Jayanna P., Petrenko V., Mao C., University of Oklahoma, US

Hydroxyapatite [(Ca5(PO4)3OH)2] is a biomineral which is associated with bone proteins to form mineralized extracellular matrix in a mature bone. Using two landscape phage libraries, in which random octa-peptides and nona-peptides are displayed on all [...]

Landscape Phage Probes for Breast Cancer Cells

Fagbohun O.A., Bedi D., Jayanna P.K., Deinnocentes P.A., Bird R.C., Petrenko V.A., Auburn University, US

Landscape phage libraries are collections of filamentous phages displaying random foreign peptides on all 4,000 surface domains of the major coat protein. They serve as a rich source of bioselective materials that are used in [...]

Landscape phage probes for PC3 prostate carcinoma cells

Jayanna P.K., Deinnocentes P., Bird R.C., Petrenko V.A., Auburn University, US

Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to [...]

Engineering hybrid nanostructures with phage particles

Makowski L., Chen L., Argonne National Laboratory, US

Assembling complex structures comprising multiple, inorganic functional elements represents a significant engineering challenge. We have constructed hybrid nanostructures based on T7 and M13 phage and incorporating a variety of metals and metal oxides. Synthesis of [...]

Engineering of bacteriophages displaying affinity tags on its head for biosensor applications

Tolba M., Brovko L.Y., Griffiths M.W., University of Guelph, CA

A method was developed for the introduction of affinity tags onto bacteriophage heads using a phage display technique. It was shown that T4 bacteriophage carrying a biotin-binding polypeptide (Biotin Carboxyl Carrier Protein, BCCP, from acetyl-CoA [...]

Landscape phage libraries as a source of bioselective nanomaterials

Kuzmicheva G.A., Sorokulova I.B., Jayanna P.K., Petrenko V.A., Auburn University, US

New generations of targeted diagnostic, therapeutic and imaging nanodevices require robust molecular recognition interfaces, which bind their targets with high specificity and selectivity. These bioselective interfaces can be formed by recombinant filamentous phages or their [...]