Papers – Page 34 – TechConnect Briefs

Dicationic gemini surfactant gene delivery complexes contain cubic-lamellar mixed polymorphic phase

Foldvari M., Wettig S., Badea I., Verrall R., Bagonluri M., University of Saskatchewan, CA

The characterization of structural properties of novel dicationic (gemini) surfactant-based DNA complexes as micro/nano-scale self-assembling delivery systems for cutaneous gene therapy is described and discussed as related to measured transfection efficiencies. Previous small-angle x-ray scattering [...]

Sensing and Plotting Single Molecule Binding Events with the Atomic Force Microscope

Johnson W.T., Molecular Imaging, US

SPM/AFM (scanning probe microscopy/atomic force microscopy) offers many unique advantages to the study of biological process at the nanometer scale. ASM/AFM allows scientists to visualize, probe, and analyze the structure of biological molecules in their [...]

Construction of Enzymosomes: Optimization of Coupling Parameters

Vale C.A., Corvo M.L., Martins L.C.D., Martins M.B.F., Marques C.R., Storm G., Cruz M.E.M., Martins L.C.D., Martins M.B.F., Laboratorio Nacional de Engenharia, Tecnologia e Inovacao, IP, PT

Different strategies can be used to improve carrier mediated delivery of therapeutic proteins. The incorporation of therapeutic enzymes into polymeric carriers [1], into aqueous space of lipid vesicles [2] or lipid-detergent vesicles [3, 4] or [...]

Functional Stimuli-Responsive Nanogel-particles for Oral Peptide Delivery: Preparation, Drug-Release Behaviors and in Vitro Cellular Interaction

Ichikawa H., Fukumori Y., Kamiya H., Kobe Gakuin University, JP

The present study involves preparation and evaluation of novel core-shell nanoparticles (CSNPs) consisting of thermo-responsive poly(N-isopropylacrylamide) (p(NIPAAm)) hydrogel core with pH-responsive poly(methacrylic acid-grafted-poly(ethylene glycol)) (p(MAA-g-EG)) shell layer as a carrier for oral peptide delivery. The [...]

Chitosan Nanoparticles for the Effective Vaccine Delivery System

Kim B.G., Kang I.J., Kyungwon University, KR

There are four genes of S, C, X, P in HV DNA. S gene has the pre-S region composed of pre-S1 and pre-S2 as an encoding gene of surface antigen. C gene is in the [...]

Synthesis and Photodynamic Efficacy of PAMAM-Photosensitiser Conjugates in Vitro

Peng C.L., Lai P.S., Lai T.-Y., Chiu C.H., Chang C-C, Lai P.S., Lai T.-Y., Shieh M.J., National Taiwan University, TW

Starburst polyamidoamine (PAMAM) dendrimer is a new class of highly branched spherical polymer. Covalent attachment of porphyrin to PAMAM dendrimer may alter the pharmacokinetics, cell targeting, and photophysical properties of photosensitizer. We investigated the uptake, [...]

Synergistic Release of DNA from pH Responsive Polyion Complexes (PICs)

Harper G., WetaScience, UK

Polyelectrolyte complexes based on an outer layer of cationic polyhydroxylamine (PT) were prepared on carboxylated microparticles pretreated with polyamine (A) and a middle layer of Polyacrylic Acid (PA). A synergistic effect between PT and PA [...]

A Novel Biodegradable System Based on BSA/PCL Core-shell Structured Nanofibers for Controlled Drug Delivery

Feng Y., Zhang Y.Z., Yong T., Ramakrishna S., National University of Singapore, SG

Electrospun core-shell structured nanofibers encapsulating BSA were explored as a potential drug delivery model in a biological system in present study. These nanofibers were prepared by coaxial electrospinning method with poly (caprolactone) (PCL) as the [...]

Microscopic Mechanism of Sonoporation

Prentice P.A., Burns J.M., Cuschieri A., Campbell P.A., University of Dundee, UK

This paper will expand on our recent paper in Nature Physics (November 2005) and will show that the initial observations can be extended from rigid albumin systems to flexible lipid based microbubbles also. We will [...]

Cationic Nanoparticles based on pH Responsive Polyion Complexes containing Plasmid GFP DNA

Harper G., Gibbs R., WetaScience, UK

Cationic nanoparticles were formulated from a polycationic Polyhydroxylamine (PT) with low pKa and polyanionic PolyAcrylic Acid (PA) that form binary polyelectrolyte complexes (PECs). Particles spontaneously form on component mixing with sizes between 80-700nm and particle [...]